Uas mir 277 sponge

Manufactured by Bloomington Drosophila Stock Center

The UAS-miR-277-sponge is a tool for manipulating the expression of miR-277 in Drosophila. It functions by sequestering miR-277, reducing its availability for targeting endogenous mRNAs.

Automatically generated - may contain errors

Lab products found in correlation

Uas cas9 p2, by Bloomington Drosophila Stock Center (1 mentions) Uas dmyc, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using uas mir 277 sponge

Transgenic Drosophila Fly Lines for Research

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following transgenic flies were employed: esg^ReDDM [3 (link)], NRE::mCherry [81 (link)], Gbe+Su(H)dsRed (T. Klein), UAS-miR-277 [25 (link)], Mex-Gal4, UAS-P35 (Bruce A. Hay), UAS-ras^G12V,UAS-p53-RNAi,UAS-apc-RNAi;UAS-Med-RNAi,UAS-Pten-RNAi [49 (link)]. Ubi-GFP and Ubi-miR-277::GFP sensor flies are kindly provided by Klaus Förstemann. From Bloomington Drosophila Stock Center (BDSC): UAS-miR-277-sponge (BL61408), UAS-CG4860-RNAi (BL67769), UAS-CG9547-RNAi (BL53327), UAS-Mtpα-RNAi (BL32873), UAS-yip2-RNAi (BL36874), UAS-CG4389-RNAi (BL32873), UAS-CG5599-RNAi (BL32876), UAS-Cas9.P2 (BL58985), vas-phiC31;attP51C (BL24482), vas-phiC31;;attP86Fb (BL24749), UAS-ras^G12V (II) (BL64195), UAS-ras^G12V (III) (BL64196), U6-EGFP-sgRNA (BL79393), UAS-CG31075-RNAi (BL50654), UAS-mCherrymitoOMM (BL66532). From Vienna Drosophila Resource Center (VDRC): UAS-N-RNAi (GD14477), CG9547::sGFP (v318106).

Zipper L., Batchu S., Kaya N.H., Antonello Z.A, & Reiff T. (2022). The MicroRNA miR-277 Controls Physiology and Pathology of the Adult Drosophila Midgut by Regulating the Expression of Fatty Acid β-Oxidation-Related Genes in Intestinal Stem Cells. Metabolites, 12(4), 315.

+ Open protocol

+ Expand

Drosophila Genetic Manipulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were obtained from the Bloomington Drosophila Stock Center: UAS-dmyc (#7118); UAS-miR-277 (#36559); UAS-miR-277^sponge (#61408). These Fly lines carrying UAS-RNA interference (RNAi) constructs were obtained from the Tsinghua Fly Center: dmyc^RNAi (#47953); imd^RNAi (#31706); Tab2^RNAi (#24667). As well as previously purchased tubulin-Gal80^ts;TM2/TM6B (#7019) and tubulin-Gal4/TM3, Sb¹, Ser¹ (#5138), all flies were raised on maize malt molasses food in a light-dark (12-hr cycle) incubator at 25°C and 60% humidity. Flies were shifted to 30°C 24 h prior to and then during infection for UAS-protein/UAS-miR-277^sponge/UAS-protein^RNAi overexpression experiments.

Li R., Zhou H., Jia C., Jin P, & Ma F. (2020). Drosophila Myc restores immune homeostasis of Imd pathway via activating miR-277 to inhibit imd/Tab2. PLoS Genetics, 16(8), e1008989.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!