The cannulas were tunneled subcutaneously to the back of the rat and, at the beginning of the experiment, the arterial cannula was connected using polyethylene tubing (PE-10 connected to a PE- 50) to a pressure transducer (Stathan P 23 Db) coupled to a multichannel recorder (Power Lab, ADInstruments). MAP and HR were recorded in conscious and unrestrained rats.
P23db
Manufactured by ADInstruments
The P23DB is a high-performance pressure transducer designed for a variety of laboratory applications. It is capable of measuring pressure within a specified range and provides a corresponding electrical output signal for data acquisition and analysis.
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Lab products found in correlation
3 protocols using p23db
1
Arterial Monitoring in Conscious Rats
2
Langendorff-Perfused Rat Heart I/R Injury
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The I/R injury model in Langendorff-perfused rat hearts was performed as previously described [22 (link)]. In brief, the rats were anesthetized with sodium pentobarbital (45 mg/kg I.P.), the hearts were rapidly resected, and the Krebs–Henseleit (K-H) solution was infused at 37 °C using a Langendorff apparatus at a constant pressure of 80 mm Hg as described in the previous paper. A water-filled latex balloon was connected to a pressure sensor (Gould P23DB, AD instrument) and inserted into the left ventricular cavity to achieve a stable LVEDP of 5–10 mmHg during the initial equilibrium. After balanced perfusion, the heart was ischemic for 30 min, followed by another 45 min of perfusion. LVDP and ±dp/dt max were evaluated using the PowerLab system (AD instrument, Dunedin, New Zealand).
3
Langendorff-Perfused Rat Heart I/R Injury Model
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The mitochondrial fusion/ ssion detection of myocardiocytes was detected as described previously [19] .
2.14 I/R injury model in Langendorff-perfused rat hearts I/R injury model in Langendorff-perfused rat hearts was performed as previously [19] . In brief, the rats were anesthetized with sodium pentobarbital (45 mg/kg I.P.), the hearts were rapidly resected-and the Krebs-Henselite (K-H) solution was infused at 37 ° C using a Langendorff apparatus at a constant pressure of 80 mm Hg as described in the previous paper. A water-lled latex balloon was connected to a pressure sensor (Gould P23DB, AD instrument) and inserted into the left ventricular cavity to achieve a stable LVEDP of 5-10mmHg during the initial equilibrium. After balanced perfusion, the heart was ischemia-free for 30 minutes, followed by another 45 minutes of perfusion. LVDP and ± dp/dt Max were evaluated using PowerLab system (AD instrument).
2.14 I/R injury model in Langendorff-perfused rat hearts I/R injury model in Langendorff-perfused rat hearts was performed as previously [19] . In brief, the rats were anesthetized with sodium pentobarbital (45 mg/kg I.P.), the hearts were rapidly resected-and the Krebs-Henselite (K-H) solution was infused at 37 ° C using a Langendorff apparatus at a constant pressure of 80 mm Hg as described in the previous paper. A water-lled latex balloon was connected to a pressure sensor (Gould P23DB, AD instrument) and inserted into the left ventricular cavity to achieve a stable LVEDP of 5-10mmHg during the initial equilibrium. After balanced perfusion, the heart was ischemia-free for 30 minutes, followed by another 45 minutes of perfusion. LVDP and ± dp/dt Max were evaluated using PowerLab system (AD instrument).
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