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Heparinized microhematocrit capillary tube

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Heparinized microhematocrit capillary tubes are small glass tubes used to collect and measure the volume percentage of red blood cells in whole blood samples. They contain a heparin coating that prevents the blood from clotting during the measurement process.

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Lab products found in correlation

Glycogen colorimetric fluorometric assay kit, by Abcam (1 mentions) Cd45.1 c57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Nsg mice, by Jackson ImmunoResearch (1 mentions) Procyte dx hematology analyzer, by IDEXX (1 mentions) Poly 1 poly c, by Cytiva (1 mentions) Aldosterone elisa kit, by Cayman Chemical (1 mentions) Micro hematocrit centrifuge, by Unico (1 mentions) Atrial natriuretic peptide eia kit, by Merck Group (1 mentions)

Close spelling variants of this product

Microhematocrit capillary tubes Heparinized capillary tubes Microhematocrit tubes Heparinized tubes Capillary tubes

5 protocols using heparinized microhematocrit capillary tube

1

Comprehensive Metabolic Profiling of Blood and Liver

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Blood was collected using Heparinized Microhematocrit Capillary Tubes (Thermo Fisher Scientific) and centrifuged at 15,000×g to obtain plasma. Blood glucose was measured using the blood glucose meter Glutest Neo Super (Sanwa Kagaku Kenkyusho Co., Ltd., Aichi, Japan). Plasma insulin levels were measured using the ultra sensitive mouse insulin ELISA Kit (Morinaga Institute of Biological Science, Inc., Kanagawa, Japan). Plasma lipoproteins were analyzed using a lipoprotein profiling service, LipoSEARCH (Skylight Biotech Inc, Akita, Japan). This analysis consists of gel filtration chromatography followed by an on-line enzymatic method for simultaneous quantification of cholesterol, TG, and PC, according to the procedure described by Usui et al. (2002) (link) with slight modifications. The nomenclature of the fractions was based on the elution pattern of control plasma that was used for quality check. Glycogen content in liver was analyzed using Glycogen Colorimetric/Fluorometric Assay Kit (BioVision, Inc., Milpitas, CA).
Hashidate-Yoshida T., Harayama T., Hishikawa D., Morimoto R., Hamano F., Tokuoka S.M., Eto M., Tamura-Nakano M., Yanobu-Takanashi R., Mukumoto Y., Kiyonari H., Okamura T., Kita Y., Shindou H, & Shimizu T. (2015). Fatty acid remodeling by LPCAT3 enriches arachidonate in phospholipid membranes and regulates triglyceride transport. eLife, 4, e06328.
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2

Genetically Modified Mouse Model Development

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All animals were housed at Memorial Sloan Kettering Cancer Center (MSK). All animal procedures were completed in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committees at MSK. Generation and genotyping of the Xpo1E571K/WT are described below.
Xpo1E571K/WT mice on a pure C57BL/6 background were crossed to CD19-cre mice (47 (link)), tamoxifen-inducible CAG-creERT mice (48 (link)), Eμ-Myc transgenic mice (31 (link)), and/or Vav-BCL2 transgenic mice (32 (link)), each of which were also maintained on a pure C57BL/6 background.
Eight-week-old female CD45.1 C57BL/6J mice (the Jackson Laboratory) were used as recipients for bone marrow transplantation assays and 8-week-old female NSG mice (the Jackson Laboratory) were used for cell-line xenografting. Blood was collected by submandibular bleeding using heparinized microhematocrit capillary tubes (Thermo Fisher Scientific). For assessment of germinal center formation, age- and sex-matched C57BL/6J mice were immunized intraperitoneally at 8 to 12 weeks old with 0.5 mL of a 2% sheep red blood cell (SRBC) suspension in PBS (Cocalico Biologicals) or PBS as control. Automated peripheral blood counts were obtained using a ProCyte Dx Hematology Analyzer (IDEXX).
Taylor J., Sendino M., Gorelick A.N., Pastore A., Chang M.T., Penson A.V., Gavrila E.I., Stewart C., Melnik E.M., Chavez F.H., Bitner L., Yoshimi A., Lee S.C., Inoue D., Liu B., Zhang X.J., Mato A.R., Dogan A., Kharas M.G., Chen Y., Wang D., Soni R.K., Hendrickson R.C., Prieto G., Rodriguez J.A., Taylor B.S, & Abdel-Wahab O. (2019). Altered nuclear export signal recognition as a driver of oncogenesis. Cancer discovery, 9(10), 1452-1467.
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3

Conditional Transgenic Mice for IDH1/2 Mutations

Cited 1 time
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The conditional Jak2V617F mice were previously described (13 (link)). Conditional transgenic IDH1R132H mice (expressing a mutation in the IDH1 gene) were provided by Kwok-Kin Wong (Dana-Farber Cancer Institute), and conditional Idh2R140Q mice were provided by Craig B. Thompson (Memorial Sloan Kettering Cancer Center). All mice were maintained on a C57BL/6J background, and the lines were backcrossed for 6 generations before use in experiments. Samples for experiments were obtained from male and female mice aged 6 months to 1.5 years. Upon induction, mice received 5 i.p. injections of polyI:polyC (Amersham) of 200 μl of a 1-mg/ml solution. Peripheral blood was collected via cheek bleeding using heparinized microhematocrit capillary tubes (Thermo Fisher Scientific). Peripheral blood counts were obtained using a HemaVet according to the manufacturer’s instructions. Excision 2 weeks after induction was confirmed using PCR.
McKenney A.S., Lau A.N., Somasundara A.V., Spitzer B., Intlekofer A.M., Ahn J., Shank K., Rapaport F.T., Patel M.A., Papalexi E., Shih A.H., Chiu A., Freinkman E., Akbay E.A., Steadman M., Nagaraja R., Yen K., Teruya-Feldstein J., Wong K.K., Rampal R., Heiden M.G., Thompson C.B, & Levine R.L. (2018). JAK2/IDH-mutant–driven myeloproliferative neoplasm is sensitive to combined targeted inhibition. The Journal of Clinical Investigation, 128(2), 789-804.
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4

Submandibular Vein Blood Sampling

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The submandibular vein was punctured using a 5.5 mm Goldenrod animal lancet (Medipoint Inc, Mineola, NY, USA). Whole blood samples were collected by using a Heparinized Microhematocrit Capillary Tube (Produce number: 22-362-566, Fisherbrand, Pittsburgh, PA, USA) and then centrifuged for 5 min in a Unico Micro-Hematocrit Centrifuge (Model: C-MH30, Dayton, NJ, USA).
Yan Y., Wang J., Chaudhry M.A., Nie Y., Sun S., Carmon J., Shah P.T., Bai F., Pratt R., Brickman C., Sodhi K., Kim J.H., Pierre S., Malhotra D., Rankin G.O., Xie Z.J., Shapiro J.I, & Liu J. (2019). Metabolic Syndrome and Salt-Sensitive Hypertension in Polygenic Obese TALLYHO/JngJ Mice: Role of Na/K-ATPase Signaling. International Journal of Molecular Sciences, 20(14), 3495.
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5

Comprehensive Urine and Serum Analysis

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Urine collected over 24hrs using metabolic cages was sent to Idexx Laboratories, Inc. for analysis of sodium, potassium, calcium, magnesium, phosphorus, creatinine, pH and glucose. Serum isolated from collected blood was sent to Idexx for analysis of sodium, potassium, calcium, magnesium, phosphorus, creatinine, and BUN. Aldosterone concentration was measured in serum using Aldosterone Elisa kit (Cayman chemicals, Catalogue #501090) as per the manufacturer’s instructions. Atrial natriuretic peptide concentration was measured in serum using Atrial Natriuretic Peptide EIA kit (Sigma, Catalogue#RAB0385). For Hematocrit measurement, tail vein was clipped and whole blood samples were collected by using a Heparinized Microhematocrit Capillary Tube (Produce number: 22-362-566, Fisherbrand, Pittsburgh, PA, USA) and then centrifuged for 5 min in a Unico Micro-Hematocrit Centrifuge (Model: C-MH30, Dayton, NJ, USA).
Baigent C., Collins R., Appleby P., Parish S., Sleight P, & Peto R. (1998). ISIS-2: 10 year survival among patients with suspected acute myocardial infarction in randomised comparison of intravenous streptokinase, oral aspirin, both, or neither. BMJ : British Medical Journal, 316(7141), 1337-1343.
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