Glacial acetic acid (Searle, Essex, England), carrageenan (Sigma-Aldrich, Laborchemikalein GmBH, Germany), piroxicam injection (Pfizer), naloxone hydrochloride (Abcam Plc, Cambrige U.K), morphine sulphate (Martindale Pharmaceuticals, U.K), Dragendorffs reagent, Chips of magnesium metal, Ferric Chloride, Wagner reagent, Sulphuric acid (BDH Ltd, poole, England), Leishman Stain, Agappe Kits (Creatinine PN 51009001, Urea PN 51216001, Sodium PN 510014001 and Potassium PN 51015002), Labcare diagnositic kits, Randox kits (Ref AST AS101) and (Ref ALT AL100) and Dolabo kits (Ref ALP AP307) were used in the study.
Naloxone hydrochloride
Manufactured by Abcam
Sourced in United Kingdom
Naloxone hydrochloride is a chemical compound used in the laboratory setting. It functions as an opioid antagonist, capable of reversing and blocking the effects of opioid drugs.
Automatically generated - may contain errors
Lab products found in correlation
Carrageenan, by Merck Group (1 mentions)
Damgo, by Abcam (1 mentions)
Axio observer z1 system, by Zeiss (1 mentions)
Way100635 maleate, by Bio-Techne (1 mentions)
Rat tnf α elisa kit, by MyBioSource (1 mentions)
Rat il 1β elisa kit, by MyBioSource (1 mentions)
Concanavalin a cona, by Thermo Fisher Scientific (1 mentions)
β actin, by Thermo Fisher Scientific (1 mentions)
Rat il 6 elisa kit, by MyBioSource (1 mentions)
4 protocols using naloxone hydrochloride
1
Comprehensive Biomedical Analysis Protocol
2
Live-cell Calcium Imaging of Neuronal Responses
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On the day of live cell calcium imaging, 50 µg Fluo-4 AM cell-permeant dye (Invitrogen) was diluted in 10 µl of 20% pluronic F-127 in DMSO. Fluo-4 AM was diluted 1:1000 in BrainPhys medium and added to coverglass chamber wells after rinsing cells once in HBSS. Samples were incubated at 37°C for 30 min. After incubation, Fluo-4 AM medium was removed, washed once in HBSS and fresh BrainPhys without phenol red was added. Samples were imaged directly on the Zeiss Axio Observer.Z1 system. Time-lapse series were acquired at 50 ms exposure using a 488 nm LED at 200 ms intervals for 1–1.5 min duration. After imaging baseline activity in BrainPhys, 1 μM DAMGO (MOR agonist, 10 mM stock in water; Abcam) was added directly to chamber wells and imaged again. The competitive opioid receptor antagonist naloxone hydrochloride was then added at 10 μM (10 mM stock in water; Abcam). EMLO-dissociated cultures, spinal sensory, and spinal motor neuron samples were imaged in succession to control for time of Fluo-4 AM cell loading. Time-lapse series were quantified in ImageJ.
3
Tramadol Hydrochloride Pharmacokinetic Protocol
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Tramadol hydrochloride (Pharmacy, UMC Groningen, Netherlands) was prepared from tablets obtained from a local pharmacy, grinded and suspended in 0.9% NaCl (saline). Naloxone hydrochloride (Abcam; Cambridge, United Kingdom) and WAY100,635 maleate (Tocris Bioscience; Bristol, United Kingdom) were dissolved in saline and each solution was freshly prepared on each testing day. All drugs were administered via intraperitoneal (IP) injection.
4
Evaluation of Anti-inflammatory Mechanisms
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Concanavalin A (con A) was purchased from Alfa Aesar, Erlenbachweg 2, Kandel, (Germany). Naloxone hydrochloride was purchased from abcam Biotechnology, Cambridge, (UK). Alanine amino transferase (ALT), aspartate amino transferase (AST), albumin, and bilirubin were purchased from Bio-Med diagnostics, (USA). Reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and thiobarbituritic acid reactive substance (TBARS) assay kits were purchased from Bio-diagnostic, (Egypt). Rat interferon-γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA) kit, rat TNF-α ELISA kit, rat IL-6 ELISA kit and rat IL-1β ELISA kit were purchased from MyBioSource, San Diego, California, (USA), Cusabio, Houston, (USA), R&D Systems, McKinley Place NE, Minneapolis, (USA) and MyBioSource, San Diego, California, (USA), respectively. An antibody raised against Nuclear factor kappa B (NF-κB p65), c-Jun N-terminal kinase (JNK), Nuclear factor erythroid 2 (Nrf2), Heme oxygenase-1 (HO-1), TLR4 and β-actin were purchased from Thermo Fisher Scientific, Waltham, (USA). Goat Anti-Rabbit HRP linked IgG and Goat Anti-Mouse HRP linked IgG were obtained from abcam Biotechnology, Cambridge, (UK).
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