Eptifibatide

G-Ro was obtained from Ambo Institute (Daejon, Korea). Thrombin was obtained from Chrono-Log Corporation (Havertown, PA, USA). Anti-VASP, anti-phosphor-VASP (Ser¹⁵⁷), anti-phosphor-VASP (Ser²³⁹), anti-rabbit IgG-HRP-horseradish peroxidase conjugate (HRP), and lysis buffer were purchased from Cell Signaling (Beverly, MA, USA). The αIIb/β₃ inhibitor eptifibatide, GR 144053, and anti-β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyvinylidene difluoride membrane was purchased from GE Healthcare (Piseataway, NJ, USA). Enhanced chemiluminescence solution was purchased from GE Healthcare (Chalfont St. Giles, UK). cAMP and cyclic guanosine monophosphate (cGMP) enzyme immunoassay kits were purchased from Cayman Chemical (Ann Arbor, MI, USA). An A-kinase inhibitor Rp-8-Br-cAMPS, an A-kinase activator 8-(4-chlorophenylthio)-cAMP (pCPT-cAMP), and a G-kinase activator 8-Br-cGMP were purchased from Sigma Chemical Corporation (St. Louis, MO, USA). Fibrinogen Alexa Fluor 488 conjugate was obtained from Invitrogen Molecular Probes (Eugene, OR, USA).

G-Ro (molecular weight=957.1) was obtained from Ambo Institute (Daejon, Korea). Thrombin was obtained from Chrono-Log Corporation (Havertown, PA, USA). Anti-PI3K, anti-phosphor-PI3K p85 (Tyr⁴⁵⁸), anti-Akt, anti-phosphor-Akt (Ser⁴⁷³), anti-rabbit IgG-horseradish peroxidase, and lysis buffer were purchased from Cell Signaling (Beverly, MA, USA). Wortmannin (PI3K inhibitor) and miltefosine (Akt inhibitor) were purchased from Cayman Chemical (Ann Arbor, MI, USA). CytoSelect 48-well cell adhesion assay kits (fibronectin-coated, colorimetric format) were purchased from Cell Biolabs (San Diego, CA, USA). Eptifibatide (αIIb/β₃ inhibitor), GR 144053 (αIIb/β₃ inhibitor), and anti-β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyvinylidene difluoride (PVDF) membrane and enhanced chemiluminesence solution (ECL) were purchased from General Electric Healthcare (Amersham, Buckinghamshire, UK). Fibrinogen Alexa Fluor 488 conjugate was obtained from Invitrogen Molecular Probes (Eugene, OR, USA).