The largest database of trusted experimental protocols

2 protocols using myc tag 9b11 mouse mab 2276s

1

Antibody Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies and fluorescent dyes used in this study were as follows: anti-Flag (FUJIFILM Wako, 018-22381), normal mouse IgG (Santa Cruz, sc-2025), and calcein AM (MB5279, Meilunbio). Anti-GAPDH (14C10) rabbit mAb (2118S), LaminA/C (4C11) mouse mAb (4777S), and Myc-tag (9B11) mouse mAb (2276S) were purchased from Cell Signaling Technology. Anti-SUN2 antibody [EPR6557] (ab124916), goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077), and goat anti-mouse IgG H&L (Alexa Fluor® 555) (ab150114) were purchased from Abcam. Peroxidase AffiniPure goat anti-mouse IgG (H+L) (115-035-003) and peroxidase AffiniPure rabbit anti-goat IgG (H+L) (305-035-003) were purchased from Jackson ImmunoResearch Laboratories. F-actin monoclonal antibody (NH3) (MA1-80729), Alexa Fluor 488 Isolectin GS-IB4 conjugate (I21411), and Hoechst 33342 solution (H3570) were purchased from Invitrogen. γ-tubulin antibody (T6557) and BSI lectin-FITC (L9381) were purchased from Sigma-Aldrich. The anti-TMEM201 rabbit antibody, generated with the TMEM201 (430–638 amino acid) peptide, was made by Youke Biological Technology Co. Ltd.
+ Open protocol
+ Expand
2

Comprehensive Antibody Reagents for cGAS-STING Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-FLAG tag rabbit polyclonal antibody (D110005), Anti-cGAS rabbit polyclonal antibody (D163570), HRP (horseradish peroxidase)-conjugated Goat Anti-Rabbit IgG (D110058) and HRP-conjugated Goat Anti-Mouse IgG (D110087) were purchased from Sangon Biotech (Shanghai, China). HA tag Polyclonal antibody (51064-2-AP) and IRF3 Polyclonal antibody (11312-1-AP) were purchased from Proteintech (Wuhan, China); STING (D2P2F) Rabbit mAb (13647S), Phospho-IRF-3 (Ser386) (E7J8G) XP® Rabbit mAb antibody (37829S) and Myc-Tag (9B11) Mouse mAb (2276S) were bought from Cell Signaling Technology. Anti-HIST3H3 Polyclonal Antibody (K106623P) were purchased from Solarbio (Beijing, China). GAPDH Mouse Monoclonal Antibody (AF5009) and β-actin Mouse Monoclonal Antibody (AA128) were purchased from Beyotime Biotechnology (Shanghai, China). Anti-HSP90β antibody was purchased from Abbkine (ABP54794) (China).
TransStart® Top Green qPCR SuperMix (+Dye II) was purchased from Transgen (Beijing, China). Cell membrane/cytoplasm/nuclear membrane protein step extraction kit (BB-31042) was bought from BestBio (Shanghai, China). Lipofectamine 3000 was purchased from invitrogen. Chemical reagents polybrene (Millipore), puromycin and RNase inhibitor (Thermo), MG132 (Beyotime), chloroquine (CQ) (tlrl-chq, InvivoGen) and ISD (tlrl-isdc, InvivoGen) were bought from indicated manufactures.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!