We used previously described protocols [63 (link), 64 (link)]. The following antibodies were used: anti-p53 (DO-1), anti-p65 (C-20) and IgG (sc-2025 or sc-2027) (Santa Cruz Biotechnology). ChIP-qPCR experiments were performed using Sybr MasterMix (Kapa Biosystems) and 2 μl of enriched DNA. Results were analyzed by the comparative Ct method (ΔCt) and normalized as % of input. Regions in the promoter of GAPDH or ACTB and p21 or MCP1 genes served as negative and positive controls, respectively. Primers were selected using Primer 3 (http://primer3.ut.ee/ ).
Igg sc 2025 or sc 2027
Manufactured by Santa Cruz Biotechnology
IgG (sc-2025 or sc-2027) is a purified immunoglobulin G (IgG) antibody. IgG is the most abundant antibody class in the human body and plays a crucial role in the adaptive immune response. The product is available in two different catalog numbers, sc-2025 and sc-2027, which may represent different formulations or packaging options.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using igg sc 2025 or sc 2027
1
ChIP-qPCR Analysis of Transcription Factors
2
ChIP Assay for p53 Enrichment
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MCF7 cells were cultured in complete medium in a 150-mm Petri dishs and when reaching 70/80 % confluence were treated for 16 h with Doxo. The procedure for crosslinking, sonication, IP and analysis followed a previously described protocol [49 (link)]. Antibodies used for ChIP assays were: p53 (DO-1) and IgG (sc-2025 or sc-2027) (Santa Cruz Biotechnology®) (Millipore). ChIP analysis was performed with the comparative Cq method (ΔΔCq) and normalized as % of input, using β-actin gene as negative control and p21 as positive control for p53 enrichment.
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