Biotin teg phosphoramidite

2’-O-Me and 2’-F phosphoramidites, universal synthesis columns (MM1–2500-1), and all ancillary RNA synthesis reagents were purchased from Bioautomation. Symmetrical branching CED phosphoramidite was obtained from ChemGenes (CLP-5215). Cyanine 5 (Cy5) phosphoramidite (10–5915), stearyl phosphoramidite (10–1979), biotin TEG phosphoramidite (10–1955), hexaethyleneglycol phosphoramidite (10–1918), TEG cholesterol phosphoramidite (10–1976), 5’-Amino-Modifier 5 (10–1905), and desalting columns (60–5010) were all purchased from Glen Research. PE anti-mouse CD19 antibody (Catalog No. 115508) and PC anti-mouse/human CD45R/B220 antibody (Catalog No. 103212) were acquired from BioLegend. MIK665 was from Selleck Chem. All other reagents were purchased from Sigma-Aldrich unless otherwise specified.

Standard solvents and reagents were purchased from either Sigma-Aldrich, Chemgenes or Alfa Aesar. 5′-O-(4,4′-Dimethoxytrityl)-5-(3-trifluoroacetylamino-1-propenyl)-2′-deoxyuridine was purchased from Hongene Biotechnology. 0.5 M (1S)-(+)-(10-Camphorsulfonyl)-oxaziridine (CSO) and biotinTEG phosphoramidite were purchased from Glen Research. Sulfo-NHS-acetate was purchased from ThermoFisher Scientific. Thin layer chromatography was carried out on silica gel 60 F₂₅₄ from either Selecto Scientific (flexible plates) or Fluka (aluminum plates). Flash chromatography was performed on Fluka silica (230–400 mesh). NMR was performed on either a Varian Inova 500 or Bruker 600 MHz instrument. ¹H NMR spectra were referenced to the signal of the solvent, and ³¹P NMR used 2% phosphoric acid as an external reference. FPLC (fast protein liquid chromatography) and HPLC (high performance liquid chromatography) were performed on an AKTA Basic System from GE Healthcare. The protein used in this investigation was human α-thrombin (Haematologic Technologies). The protein was handled according to the manufacturer's recommendations and aliquots were stored at −80°C. The aptamers were stored at −20°C.