Anti ceacam1 mab

Manufactured by BioLegend

Sourced in United States

Anti-CEACAM1 mAb is a monoclonal antibody that specifically binds to the CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1) protein. CEACAM1 is a cell surface glycoprotein involved in cell-cell adhesion and cell signaling processes.

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Lab products found in correlation

Mouse igg1 isotype control, by BioLegend (1 mentions) Rat igg2a isotype control, by BioXCell (1 mentions) Anti cd4, by BioXCell (1 mentions) Anti mouse cd4, by Thermo Fisher Scientific (1 mentions) Anti mouse cd3e, by Thermo Fisher Scientific (1 mentions) Anti mouse cd25, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti ceacam1 mab

CEACAM1 and SHP2 Intracellular Staining

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Staining for CEACAM1 was conducted by FACS staining for human CEACAM1 with anti-CEACAM1 mAb (Biolegend). Intracellular staining for SHP2 was conducted using an anti-SHP2 antibody (Santa Cruz). Intracellular FACS staining for SHP2 was based on methanol fixation protocol. Briefly, cells were perforated in methanol at -20°C, rehydrated in PBS for half an hour, and then stained with antibodies in 5% BSA in PBS at room temperature.

Diab M., Vitenshtein A., Drori Y., Yamin R., Danziger O., Zamostiano R., Mandelboim M., Bacharach E, & Mandelboim O. (2016). Suppression of human metapneumovirus (HMPV) infection by the innate sensing gene CEACAM1. Oncotarget, 7(41), 66468-66479.

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Therapeutic Antibody Characterization in Mice

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Therapeutic antibodies were as follows: Anti-Tim-3 mAb (Clone RMT3-23; Catalog#: BE0115; BioXcell, West Lebanon, NH, USA), Rat IgG2a isotype control (Clone 2A3; Catalog#: BE0089; BioXcell), anti-CEACAM1 mAb (Clone MAb-CC1; Catalog#: 134504; Biolegend, Santa Cruz, CA, USA), and Mouse IgG1 isotype control (Clone MOPC-21; Catalog#: 400153; Biolegend). Antibodies for flow cytometry were as follows: anti-mouse CD3e (Catalog#: 15-0031-82; eBioscience, San Diego, CA, USA), anti-mouse CD4 (Catalog#: 12-0041-82; eBioscience), anti-mouse CD8a (Clone 53-6.7; Catalog#: 11-0081-82; eBioscience), anti-mouse CD25 (Catalog#: 25-0251-82; eBioscience), and anti-mouse Foxp3 (Catalog#: 11-5773-82; eBioscience). Antibodies for depletion were as follows: anti-CD4 (Clone GK1.5; Catalog#: BE0003-1; BioXcell) and anti-CD8 (Clone 2.43; Catalog#: BE0061; BioXcell).

Li J., Liu X., Duan Y., Liu Y., Wang H., Lian S., Zhuang G, & Fan Y. (2017). Combined Blockade of T Cell Immunoglobulin and Mucin Domain 3 and Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Results in Durable Therapeutic Efficacy in Mice with Intracranial Gliomas. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 23, 3593-3602.

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