Non-duplicate P. aeruginosa isolates were collected from clinics and hospitals from the four district municipalities. Specimens included throat swabs, wound swabs, swabs from abscesses, sputum, urine, blood culture and catheter tips. Demographic characteristics of patients and medical histories were collected from medical records including date of specimen collection, gender and age. All samples were routinely cultured on MacConkey and Blood agar plates. Blood and sputum were also cultured on chocolate agar. Suspected colonies were plated on Cetrimide agar and identified by gram staining, colony characteristics, motility, pyocyanin production and characteristics grape-like odour41 (link). Strains were identified to the species level with Vitek 2 GN (bioMérieux, Inc. USA) ID cards and confirmed by Microscan NID 2 panels (Beckman Coulter, Inc. USA). Specific primers and probes targeting gyrB were amplified by singleplex rPCR and were also used to confirm identity of the isolates.
Microscan nid 2 panels
Manufactured by Beckman Coulter
Sourced in United States
The Microscan NID 2 panels are a laboratory equipment product designed for the identification and antibiotic susceptibility testing of microorganisms. The panels provide automated analysis of bacterial cultures, delivering results to aid in the diagnosis and treatment of infectious diseases.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using microscan nid 2 panels
1
Isolating and Identifying Pseudomonas aeruginosa
2
Isolation and Identification of P. aeruginosa
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Non-duplicate P. aeruginosa isolates were collected from Mthatha, other clinics and hospitals from the four district municipalities. Specimens included throat swabs, wound swabs, swabs from abscesses, sputum, urine, blood culture and catheter tips. Demographic characteristics of patients and medical histories were collected from medical records including date of specimen collection, gender, age, test ordered and hospital/clinic. All samples were routinely cultured on MacConkey and Blood agar plates. Blood and sputum were also cultured on chocolate agar. Suspected colonies were plated on Cetrimide agar and identi ed by gram staining, colony characteristics, motility, pyocyanin production and characteristics grape-like odour (30, 31) . Strains were identi ed to the species level with Vitek 2 GN (bioMérieux, Inc. USA) ID cards and con rmed by Microscan NID 2 panels (Beckman Coulter, Inc. USA).
3
Isolation and Identification of P. aeruginosa
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Non-duplicate P. aeruginosa isolates were collected from Mthatha, other clinics and hospitals from the four district municipalities. Specimens included throat swabs, wound swabs, swabs from abscesses, sputum, urine, blood culture and catheter tips. Demographic characteristics of patients and medical histories were collected from medical records including date of specimen collection, gender, age, test ordered and hospital/clinic. All samples were routinely cultured on MacConkey and Blood agar plates. Blood and sputum were also cultured on chocolate agar. Suspected colonies were plated on Cetrimide agar and identi ed by gram staining, colony characteristics, motility, pyocyanin production and characteristics grape-like odour (30, 31) . Strains were identi ed to the species level with Vitek 2 GN (bioMérieux, Inc. USA) ID cards and con rmed by Microscan NID 2 panels (Beckman Coulter, Inc. USA).
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