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Mab22591

Manufactured by R&D Systems
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MAB22591 is a monoclonal antibody designed for the detection of an unspecified target. It is intended for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab31261, by Abcam (2 mentions) T9026, by Merck Group (1 mentions) A5545, by Merck Group (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Lysosensor yellow blue dnd 160, by Thermo Fisher Scientific (1 mentions) Spermidine, by Merck Group (1 mentions) Spermine, by Merck Group (1 mentions) N acetylcysteine amide, by Merck Group (1 mentions) Bishexamethylene triamine, by Merck Group (1 mentions) Putrescine, by Merck Group (1 mentions) Ab14748, by Abcam (1 mentions) Ab30687, by Abcam (1 mentions) N 2 mercaptopropionyl glycine, by Merck Group (1 mentions) Ir dye 700 800, by Rockland Immunochemicals (1 mentions)

2 protocols using mab22591

1

Antibody Validation for Drosophila Studies

Cited 2 times
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The following antibodies were used in this study: Mouse anti-Drosophila Rab7 hybridoma supernatant (1:5) and goat anti-Drosophila golgin245 (both developed and validated by S. Munroe [Riedel et al., 2016 (link)] and obtained from the Developmental Studies Hybridoma Bank). Rabbit anti-Drosophila Rab5 (1: 250, Ab31261; Abcam). Rat anti-tdTomato (1:500, EST203; Kerafast). Mouse anti-GFP (1:500, 1181446000; Sigma-Aldrich). Mouse anti-insect cathepsin L antibody (1:500, MAB22591; R&D Systems). Mouse anti-tubulin antibody (1:2,000, T9026; Sigma-Aldrich). The guinea pig anti-Drosophila scattered (Vps54) antibody was generated by and obtained from R. Sinka (Fári et al., 2016 (link); 1:400). Secondary antibodies for immunohistochemistry were anti-mouse, goat, rabbit, or rat labeled by Alexa 488, 555, or 647 (1:1,000; Thermo Fisher Scientific). Secondary antibodies for Western blot were anti-mouse HRP (1:1,000, 115-035-146; Jackson), anti-guinea pig HRP (1:500, A5545; Sigma-Aldrich), or anti-mouse IR-Dye 680 LT (1:20,000; LI-COR).
O’Brien C.E., Younger S.H., Jan L.Y, & Jan Y.N. (2022). The GARP complex prevents sterol accumulation at the trans-Golgi network during dendrite remodeling. The Journal of Cell Biology, 222(1), e202112108.
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2

Antibodies and Chemicals for Lysosome Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following commercially available antibodies were used: anti-Rab5 (ab31261, abcam), anti-GABARAP for Drosophila Atg8a (PM037, MBL), anti-Drosophila LAMP1 (ab30687, abcam), anti-human LAMP1 (AB2296838, DSHB), anti-cathepsin D (sc-6487, Santa Cruz), anti-Brp (AB2314866, DSHB), anti-cathepsin L (MAB 22591, R&D systems)44 (link),63 , anti-Ref(2)P28, anti-elav (AB528217, DSHB), anti-ATP5α (ab14748, abcam), Cy5-conjugated anti-HRP (123175021, Jackson ImmunoLab), and secondary antibodies conjugated to Alexa 488/568/647 (Thermo Fisher Scientific), or near infrared (IR) dye 700/800 (Rockland). The following chemicals were used in the study: N-acetylcysteine amide (A0737, Sigma-Aldrich), N-(2-mercaptopropionyl)glycine (M6635, Sigma-Aldrich), putrescine (51799, Sigma-Aldrich), spermidine (S0266, Sigma-Aldrich), spermine (s4264, Sigma-Aldrich), bis(hexamethylene)triamine (421960, Sigma-Aldrich), DHE (D11347, Thermo Fisher Scientific), LysoTracker® Red DND-99 (L7528, Thermo Fisher Scientific), LysoSensor™ Yellow/Blue DND-160 (L7545, Thermo Fisher Scientific).
Li C., Brazill J.M., Liu S., Bello C., Zhu Y., Morimoto M., Cascio L., Pauly R., Diaz-Perez Z., Malicdan M.C., Wang H., Boccuto L., Schwartz C.E., Gahl W.A., Boerkoel C.F, & Zhai R.G. (2017). Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome. Nature Communications, 8, 1257.
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