Ph3 ser10

Manufactured by Merck Group

The PH3 Ser10 is a laboratory equipment product manufactured by Merck Group. It is designed to measure and analyze the pH levels of various samples. The core function of this product is to provide accurate and reliable pH measurements to support scientific research and analysis.

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Lab products found in correlation

2 protocols using ph3 ser10

Immunostaining of Heart Cryosections

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Immunostaining was performed according to prior reports^{[1 (link)]}. Briefly, heart cryosections were equilibrated with antigen retrieval buffer in epitope retrieval buffer (IHC World) or 1× citrate buffer (Antigen Retrieval Citra Plus, Biogenex). Samples were permeabilized and blocked with 0.3% Triton X-100 and 10% serum from the host animal of secondary antibodies in PBS for 1 h at room temperature. Then, the samples were incubated overnight at 4 0C with primary antibodies. After three washes in PBS, samples were incubated at room temperature for 1h with the corresponding fluorescence secondary antibodies conjugated to Alexa Fluor 488 or 555 (Invitrogen) at 1:400. The slides were mounted in Vectashield Antifade Mounting Medium (Vector Laboratories). Slides were viewed under Nikon fluorescence or Zeiss LSM 510 confocal microscopes. Primary antibodies: pH3 Ser10 (EMD Millipore, 06–570; 1:100); troponin T (Thermo Scientific, MS-295-P1; 1:200); 8-oxoG (Abcam ab64548, 1:25). DAPI was used for nuclear staining. Images were obtained on a Nikon Eclipse Ni or Nikon A1 laser scanning confocal microscopes.

Ali S.R., Nguyen N.U., Menendez-Montes I., Hsu C.C., Elhelaly W., Lam N.T., Li S., Elnwasany A., Nakada Y., Thet S., Foo R.S, & Sadek H.A. (2024). Hypoxia-induced stabilization of HIF2A promotes cardiomyocyte proliferation by attenuating DNA damage. The journal of cardiovascular aging, 4(1), 11.

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Antibody Sources for AR and Associated Factors

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The sources for the antibodies and control IgGs were as following: AR (Santa Cruz, Cat. sc-816); pAR-S81 (EMD Millipore, Cat. 07–1375); pAR-S308 (Santa Cruz, Cat. sc-26406); pRNA Pol II Ser2 (Abcam, Cat., ab5095); pRNA Pol II Ser5 (Abcam, Cat. ab5131); CDK1 (Cell Signaling, Cat. 9112); pCDK1-T161 (Cell Signaling, Cat. 9114); CDK9 (Santa Cruz, Cat. sc-8338); Cyclin T1 (Santa Cruz, Cat. sc-10750); BRD4 (Bethyl, Cat. A301-985A); p300 (Santa Cruz, Cat. sc-585); Histone 3 (Abcam, Cat. ab1791); H3K27Ac (Abcam, Cat. ab4729); pH3-Ser10 (EMD Millipore, Cat. 06–570); FoxA1 (Abcam, Cat. Ab23738); PSA (Meridian Life Science, Cat. K92110R); Flag-M2 (Sigma-Aldrich, Cat. F3165); HA (Cell Signaling, Cat. 3724); β-Tubulin (EMD Millipore, Cat. MAB3408); β-Actin (Abcam, Cat. Ab6276); GAPDH (Abcam, Cat. Ab9485); Hsp90 (Santa Cruz, Cat. sc-69703), PP1 (Santa Cruz: Cat. sc-443; Cat. sc-6104; and Cat. sc-6105), and control IgGs (Santa Cruz: normal rabbit IgG, Cat. sc-2027; and normal goal IgG, Cat. sc-2028). Protein A and protein G was from Pierce (Cat. 20334 and Cat. 20399, respectively). The anti-flag M2 affinity gel was from Sigma-Aldrich (Cat. A2220). Western blots were developed using X-Ray film (Research Products International) and the Western Lightning Plus-ECL reagent (PerkinElmer). Images were acquired using a CanoScan LiDE 210 scanner.

Liu X., Gao Y., Ye H., Gerrin S., Ma F., Wu Y., Zhang T., Russo J., Cai C., Yuan X., Liu J., Chen S, & Balk S.P. (2017). Positive feedback loop mediated by protein phosphatase 1α mobilization of P-TEFb and basal CDK1 drives androgen receptor in prostate cancer. Nucleic Acids Research, 45(7), 3738-3751.

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