Anti human β actin

Manufactured by Affinity Biosciences

Sourced in United States

The Anti-human β-actin is a primary antibody that specifically binds to the β-actin protein, which is a key structural component found in the cytoskeleton of eukaryotic cells. This antibody can be used for the detection and quantification of β-actin in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab210702, by Abcam (1 mentions) Ab231303, by Abcam (1 mentions) Ab207608, by Abcam (1 mentions) Anti human n cadherin, by Abcam (1 mentions) Anti human e cadherin, by Abcam (1 mentions) Af7022, by Affinity Biosciences (1 mentions) Af5228, by Affinity Biosciences (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Bca assay, by Beyotime (1 mentions)

2 protocols using anti human β actin

Western Blotting Antibody Validation

Cited 1 time

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Western blotting experiments were performed as previously described
[24] (link). The antibodies used in this research were as follows: anti-human β-actin (AF7018; Affinity Biosciences, Cincinnati, USA), anti-human G6PD (ab210702; Abcam, Cambridge, UK), anti-human TP53 (9282; Cell Signaling Technology, Boston, USA), anti-human E-cadherin (ab231303; Abcam), anti-human N-cadherin (ab207608; Abcam), anti-human MMP-9 (AF5228; Affinity Biosciences), anti-human Bcl-2 (AF6139; Affinity Biosciences), anti-human cleaved caspase-3 (AF7022; Affinity Biosciences), anti-human Cyt-C (AF0146; Affinity Biosciences), and anti-human YY1 (ABP60802; Abbkine, Wuhan, China).

Wu F., Zhang W., Wei H., Ma H., Leng G, & Zhang Y. (2023). lncRNA ELFN1-AS1 promotes proliferation, migration and invasion and suppresses apoptosis in colorectal cancer cells by enhancing G6PD activity : lncRNA ELFN1-AS1 promotes the progression of colorectal cancer. Acta Biochimica et Biophysica Sinica, 55(4), 649-660.

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Western Blot Analysis of K562 Cells

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The K562 cell proteins were lysed with radio-immunoprecipitation assay buffer and the protein concentrations were determined by BCA assay (Beyotime Institute of Biotechnology). Equal amounts of protein were separated by the SDS polyacrylamide gel. The proteins were transferred to polyvinylidenedifluoride membranes (Millipore) and blocked with 5% skim milk. The membranes were incubated overnight with anti-human HSPA1A (Proteintech), anti-human HNRNPH3 (Santa Cruz), or anti-human β-Actin (Affinity) antibodies. After washing, the membranes were incubated with the appropriate secondary antibody (Santa Cruz) for visualizing proteins by chemiluminescence reagent. The intensity of the Western blot bands were quantified by Image J software.

Liu M., Sun X., Zhu L., Zhu M., Deng K., Nie X., Mo H., Du T., Huang B., Hu L., Liang L., Wang D., Luo Y., Yi J., Zhang J., Zhong X., Cao C, & Chen H. (2021). Long Noncoding RNA RP11-115N4.1 Promotes Inflammatory Responses by Interacting With HNRNPH3 and Enhancing the Transcription of HSP70 in Unexplained Recurrent Spontaneous Abortion. Frontiers in Immunology, 12, 717785.

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