The following mouse monoclonal anti-human antibodies were used for flow cytometry experiments: fluorescein isothiocyanate (FITC)-conjugated CD1b (clone M-T101), CD95 (clone DX2), and HLA-DPDQDR (clone Tu39); PE-conjugated CD119 (clone GIR-208), CD124 (clone hIL4R-M57), and CD132 (clone AG184); APC-conjugated CD1a (clone HI149), CD40 (clone 5C3), CD49d (clone 9F10), and HLA-ABC (clone G46-2.6; BD Biosciences, San Jose, CA, USA); FITC-conjugated CD54 (clone RR1/1); PE-conjugated CD147 (clone 8D12) (eBioscience, San Diego, CA, USA). The following isotype-matched control antibodies were also included in all experiments: FITC-conjugated mouse IgG1 (clone MOPC-21), IgG2a (clone G155-178); PE-conjugated mouse IgG1 (clone MOPC-21); and APC-conjugated mouse IgG1 (clone MOPC-21) (BD Biosciences).
Hla abc clone g46 2.6
Manufactured by BD
Sourced in United States
The HLA-ABC (clone G46-2.6) is a laboratory product used for the detection and analysis of human leukocyte antigen (HLA) class I molecules. It functions as a reagent for flow cytometry or other immunoassay applications. The product's core function is to enable the identification and quantification of HLA-ABC expression on cell samples.
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Lab products found in correlation
Apc conjugated mouse igg1 clone mopc 21, by BD (1 mentions)
Cd132, by BD (1 mentions)
Cd49d clone 9f10, by BD (1 mentions)
Tryple, by Thermo Fisher Scientific (1 mentions)
Lsr 2 cell analyser, by BD (1 mentions)
Live dead fixable yellow dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
2 protocols using hla abc clone g46 2.6
1
Comprehensive Immune Phenotyping by Flow Cytometry
2
Quantification of HLA Expression
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Cells were seeded in 24-well plates and treated the next day with indicated treatments. After 72 h, the adherent cells were lifted using Tryple (Gibco) and stained with HLA-ABC (clone G46-2.6, 1:200 dilution, 555553, BD Biosciences), HLA-DR (clone LT43, 1:200 dilution, 502515, BD Biosciences) and a viability marker (1:400 dilution, LIVE/DEAD™ Fixable Yellow Dead cell stain kit, ThermoFisher Scientific) before resuspended in 4% paraformaldehyde (Sigma-Aldrich). Cells were analysed using the BD LSR II cell analyser. FACS data was analysed using FlowJo version 10 software (Tree Star Inc., USA). All experiments were performed in triplicate with three independent experiments. The gating strategy is provided in Supplementary Fig. 9a .
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