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Model orcaflash

Manufactured by Hamamatsu Photonics
Sourced in United Kingdom

The OrcaFlash is a scientific camera designed for high-performance imaging applications. It features a high-sensitivity CMOS image sensor and advanced electronic capabilities to capture detailed images and video. The OrcaFlash is suitable for a variety of scientific research and industrial applications that require precise, high-quality imaging.

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2 protocols using model orcaflash

1

Patch-clamp Recording of Neuronal Cells

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Whole-cell patch-clamp recordings were performed under visual control using oblique illumination on a Slicecope Pro2000 (Scientifica, Uckfield, East Sussex, UK) equipped with a 12-bit monochrome CMOS camera (Hamamatsu Model OrcaFlash). Borosilicate glass pipettes (Sutter Instrument BF100-58-10) with resistances ranging from 3–7 MΩ were pulled using a laser micropipette puller (Sutter Instrument Model P-2000). Pipettes were filled using a standard intracellular solution (in mM: 135 potassium gluconate, 4 KCl, 2 NaCl, 10 HEPES, 4 EGTA, 4 Mg-ATP, 0.3 Na-GTP; 280 mOsm kg−1; pH adjusted to 7.3 with KOH). Whole-cell voltage-clamp recordings were performed using a MultiClamp 700B amplifier, filtered at 8 kHz and digitized at 20 kHz using a Digidata 1550A digitizer (Molecular Devices).
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2

Whole-cell Patch-clamp Electrophysiology

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Whole-cell patch-clamp recordings were performed under visual control using oblique illumination on a Slicecope Pro2000 (Scientifica) equipped with a 12-bit monochrome CMOS camera (Hamamatsu Model OrcaFlash). Borosilicate glass pipettes (Sutter Instrument BF100-58-10) with resistances ranging from 3-7 MΩ were pulled using a laser micropipette puller (Sutter Instrument Model P-2000) . Pipettes were filled using standard intracellular solution (in mM: 135 potassium gluconate, 4 KCl, 2 NaCl, 10 HEPES, 4 EGTA, 4 Mg-ATP, 0.3 Na-GTP; 280 mOsm kg!"; pH adjusted to 7.3 with KOH) Whole-cell voltage-clamp recordings were performed using a MultiClamp 700B amplifier, filtered at 8 kHz and digitized at 20 kHz using a Digidata 1550A digitizer (Molecular Devices).
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