N2a cells (ATCC® CCL-131TM) originated from murine neuroblastomas were maintained in DMEM culture medium “Dulbecco’s modified Eagles medium” (Gibco®) supplemented with 10% Fetal Bovine Serum (FBS) (Invivocell®), 4 mM of l -glutamine (Sigma®), 100 units/mL penicillin and 100 µg/mL streptomycin at 37 °C in 5% CO2. The cells were kept under these conditions until they reached 80% confluence, where they were either transferred to a new bottle containing new medium or seeded in plates for the assays.
Ccl 131tm
Manufactured by American Type Culture Collection
Sourced in United States
The CCL-131TM is a laboratory equipment product offered by American Type Culture Collection. It is a cell line designed for research purposes. The core function of the CCL-131TM is to provide a reliable and consistent cell culture system for various experimental applications.
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Lab products found in correlation
4 protocols using ccl 131tm
1
Murine Neuroblastoma Cell Culture
2
Oxygen-Glucose Deprivation Induces N2a Cell I/R Injury
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The N2a cell line was obtained from ATCC (CCL-131TM). DMEM containing 10% fetal bovine serum was used to treat N2a cells. To induce N2a cell I/R injury, cells were cultured in an airtight chamber containing 95% N2 and 5% CO2 for 30 minutes to induce oxygen-glucose deficiency, and then the chamber was sealed with normal oxygen for four hours to induce reoxygenation injury 35 (link). AEBSF (3 μM, cat. no. S7378, Selleck) was used to inhibit the UPRmt in N2a cells. Carbenoxolone (5 mM, cat. no. S4368, Selleck) was used to inhibit the Foxo3 pathway in N2a cells.
3
Culturing Neuro-2a Mouse Neuroblastoma Cells
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Neuro-2a (N2a) mouse neuroblastoma cells were purchased from ATCC (CCL-131TM) (Manassas, Virginia, USA). Cells were cultured typically in Dulbecco’s modified Eagle medium with high glucose (4.5 g/L) (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 units/mL Penicillin and 100 µg/mL Streptomycin and 1% GlutaMAX, at 37 °C in a humidified atmosphere with 5% CO2. The cells were passaged at 90–95% confluence at a 1:20 splitting ratio.
4
Differentiation of Neuro2a Cells using Retinoic Acid
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Neuro2a cells, a mouse neuroblastoma cell line, were purchased from ATCC (CCL-131TM). They were cultured at 37°C in MEM, supplemented with 10% FBS, 100 IU/mL penicillin, 100 µg/mL streptomycin, 0.25 mg/mL sodium bicarbonate, and 2.5 µg/mL fungizone, in a humidified incubator containing 5% CO2. Neuro2a cells were seeded at a density of 1.5 × 103 cells/cm2 in a 75 cm2 flask in MEM medium with 10% FBS and then passed at approximately 80% confluence. After that, Neuro2a cells had to differentiate to express characteristics of neuron cells by retinoic acid before conducting further tests [47 (link)–49 (link)]. It was accomplished by seeding cells in DMEM with 2% FBS and changing medium every two days until six days passed. From day 0 to day 2, we used DMEM with 2% FBS and all-trans retinoic acid (10 µM). After that, we used DMEM supplemented with 2% FBS, all-trans retinoic acid (10 µM), and cytosine arabinoside hydrochloride (0.5 µM/mL) from day 2 to day 4. After four days, the medium was replaced by DMEM containing 2% FBS and retinoic acid (10 µM) until the end of the 6th day.
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