Mitoxantrone dihydrochloride, NaTC, NaTDC and the spin probes 5-DSA, 12-DSA and 16-DSA were analytical grade and supplied by Sigma Aldrich (St. Louis, MO, USA). All the compounds were used without further purification. Experiments were performed in 0.1 M phosphate buffer (pH 7.4) and 0.1 M carbonate buffer (pH 10) and deionized water (18.2 MΩcm, Mili-Q water purification system) was used for the preparation of solutions.
Natdc
Manufactured by Merck Group
Sourced in United States
NaTDC is a laboratory reagent used in various applications. It is the sodium salt of taurodeoxycholic acid, a bile acid derivative. NaTDC is commonly used as a surfactant and detergent in biological and biochemical research. Its core function is to facilitate the solubilization and extraction of compounds, as well as to disrupt cellular membranes.
Automatically generated - may contain errors
Lab products found in correlation
Mitoxantrone dihydrochloride, by Merck Group (1 mentions)
16 dsa, by Merck Group (1 mentions)
5 dsa, by Merck Group (1 mentions)
Sodium glycodeoxycholate, by Merck Group (1 mentions)
Milli q grade water, by Merck Group (1 mentions)
Chloroform chcl3, by Thermo Fisher Scientific (1 mentions)
Ethanol etoh, by Merck Group (1 mentions)
Nacdc, by Merck Group (1 mentions)
Natcdc, by Merck Group (1 mentions)
N acetyl cysteine (nac), by Merck Group (1 mentions)
3 protocols using natdc
1
Mitoxantrone-based Liposome Characterization
2
Lipid and Bile Salt Characterization
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Materials DPPC (Figure S1A) and 1,2-dipalmitoyl-d62-sn-glycero-3-phosphocholine-1,1,2,2-d4-N,N,Ntrimethyl-d9 (d75-DPPC) (Figure S1B) were provided by Avanti Polar Lipids, Inc. (Alabaster, AL, USA), and chloroform (CHCl3) by Fisher Scientific (Loughborough, UK). NaTC (P97.0% TLC) (Figure 1A), NaTDC (P95.0% TLC) (Figure 1B), sodium glycodeoxycholate (NaGDC, P97.0% HPLC) (Figure S2) and ethanol (EtOH, P99.8% GC) were all purchased from Sigma-Aldrich (Gillingham, UK). Ultrapure water, or MilliQ-grade water (H2O, 18.2 MΩ•cm, Merck Millipore, Molsheim, France), was used in all experiments, except for the NR measurements, where deuterium oxide (D2O, P99.9%), provided by Euriso-top SAS (St. Aubin, France), and aircontrast-matched water (ACMW, 8.1% D2O / 91.9% H2O by volume) were employed. All reagents were used as supplied.
3
Peptide Synthesis and Purification
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Peptides LDQW (560.26 Da), LQKW (573.33 Da), and INYW (594.28 Da) were synthesized by Genosphere Biotechnologies (Paris, France). Purity of each peptide was further improved (final purity upper than 97%) by reversed-phase high-performance liquid chromatography as described in previous study (Sadat et al., 2011) . Pure bile salt (NaC, NaCDC, NaDC, NaGC, NaTC, NaTCDC, NaTDC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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