Multidimensional acquisitions were performed on an automated inverted microscope (Nikon Ti2 Eclipse, Nikon) using 20× objective (Nikon CFI Plan Apo 20X/0.75 Ph DM). Microscope was equipped with thermal, CO2, and humidity control and controlled using NIS software and perfect focus system. Images were obtained every 15 min over 50 hours. Up to 15 independent patterns were imaged in parallel using a motorized XY stage. Patterns where cells formed full monolayers at the start of experiments were selected for imaging.
Cfi plan apo 20x 0.75 ph dm
Manufactured by Nikon
The CFI Plan Apo 20X/0.75 Ph DM is a high-performance microscope objective lens manufactured by Nikon. It features a 20x magnification and a numerical aperture of 0.75, providing excellent image quality and resolution. The lens is designed for use in a variety of microscopy applications.
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Lab products found in correlation
2 protocols using cfi plan apo 20x 0.75 ph dm
1
Long-term Live Cell Imaging
2
Fluorescent and Confocal Microscopy Protocols
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Fluorescent images of the patterns were acquired with an inverted microscope (Nikon Ti2 Eclipse, Nikon) with an objective 20×/0.75 (Nikon CFI Plan Apo 20X/0.75 Ph DM). Confocal images were taken using inverted confocal microscope Axio Observer 7 (Spectral Detection Zeiss LSM 800) using 40×/1.3 Oil DIC M27 or 63×/1.4 Oil DIC M27 objectives with ZEN 2.3 imaging software. For integrin imaging, Zeiss Axiovert 200M microscope was used with 20× and 40× objective. For pMLC2 imaging, Zeiss LSM 780 microscope was used with 20×/0.8 M27 Plan-Apochromat and 40×/1.20 W Korr M27 C-Apochromat objectives using ZEN 2.1 SP3 software.
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