A 100 µl cell suspension containing 1 × 10 5 cells was prepared. Then, 5 µl annexin V-FITC and 10 µl propidium iodide (20 µg/ml) were added, and cells were incubated for 15 min in the dark at room temperature. The percentage of apoptotic cells was analyzed using a FACS Caliber ow cytometer (BD Biosciences, USA). Each experiment was repeated three times.
Facs caliber ow cytometer
Manufactured by BD
Sourced in United States, Germany
The BD FACS Caliber is a flow cytometer designed for quantitative analysis of cells and particles. It utilizes laser technology to detect and measure various physical and fluorescent properties of cells as they pass through a flow cell. The FACS Caliber is capable of simultaneously analyzing multiple parameters of individual cells within a sample.
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Lab products found in correlation
3 protocols using facs caliber ow cytometer
1
Annexin V-FITC Apoptosis Assay
2
Annexin V-FITC/PI Apoptosis Assay
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Cells were harvested and resuspended in 500 μl of binding buffer, and stained with Annexin V-FITC/PI Apoptosis kit (BD Biosciences). Apoptosis percentages were then detected using a FACSCaliber ow cytometer (BD Biosciences) and analyzed by Flowjo V10.1.
3
Flow Cytometry Analysis of CD34 and CD44 Markers
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All of the groups with different treatments for 24 h were assessed by ow cytometry for expressing CD34 and CD44 marker protein. Cell suspensions of the B16F10 cell line were incubated with 1 μL ofCD44 Rat anti-Human,Mouse PE, Clone (ebioscience Ins-sandiego, USA), and anti-mouse CD34 FITC conjugated (ebioscience Ins-sandiego, USA),individually.Anti Rat IgG2b-PE isotype control for CD44, Rat IgG2a K isotype control FITC for CD34, and unstained cells were used as controls. The phenotype was assessed using a FACS caliber ow cytometer (BD, Heidelberg, Germany).
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