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Stevioside

Manufactured by Fujifilm
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Sourced in Germany

Stevioside is a laboratory equipment product manufactured by Fujifilm. It is a natural sweetener extracted from the leaves of the Stevia rebaudiana plant. Stevioside is used as a reference standard in the analysis and quantification of steviol glycosides, which are the primary sweet compounds found in Stevia plant extracts.

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Lab products found in correlation

Calcium carbonate, by Merck Group (1 mentions) Coniferyl alcohol, by Merck Group (1 mentions) Neutrase 0.8 l enzyme, by Novozymes (1 mentions) Potato dextrose agar pda, by BD (1 mentions) Methanol, by Merck Group (1 mentions) Sodium alginate, by Merck Group (1 mentions) Ferulic acid, by Merck Group (1 mentions) Potato dextrose broth (pdb), by BD (1 mentions) Potato dextrose agar (pda), by BD (1 mentions) Tween 20, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions)

2 protocols using stevioside

1

Silybins Precursors Extraction from Milk Thistle

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The specimens of S. marianum under study were collected in the banks of Carrión river as it passes through the town of Palencia (Spain) during stage 67 (or 6N7) according to the extended BBCH scale [34 (link)]. This stage was chosen because silybins precursors (Figure S1) should not have yet been consumed. The capitula of S. marianum were shade-dried and pulverized to fine powder in a mechanical grinder. Different specimens (n = 25) were thoroughly mixed to obtain a composite sample.
Chitosan (CAS 9012-76-4; high MW: 310,000–375,000 Da) was supplied by Hangzhou Simit Chem. and Tech. Co. (Hangzhou, China). NeutraseTM 0.8 L enzyme was supplied by Novozymes A/S (Bagsværd, Denmark). Stevioside (CAS 57817-89-7, 99%) was purchased from Wako Chemicals GmbH (Neuss, Germany). Coniferyl alcohol (CAS 458-35-5, 98%), ferulic acid (CAS 537-98-4, European Pharmacopoeia Reference Standard), sodium alginate (CAS 9005-38-3), calcium carbonate (CAS 471-34-1, ≥99.0%), and methanol (CAS 67-56-1, UHPLC, suitable for mass spectrometry) were acquired from Sigma-Aldrich Química (Madrid, Spain). PDA (potato dextrose agar) was supplied by Becton Dickinson (Bergen County, NJ, USA).
The three fungal pathogens (Table 1) were supplied as lyophilized vials (later reconstituted and refreshed as PDA subcultures) by the Agricultural Technological Institute of Castilla and Leon (ITACYL, Valladolid, Spain) [35 ].
Langa-Lomba N., Buzón-Durán L., Sánchez-Hernández E., Martín-Ramos P., Casanova-Gascón J., Martín-Gil J, & González-García V. (2021). Antifungal Activity against Botryosphaeriaceae Fungi of the Hydro-Methanolic Extract of Silybum marianum Capitula Conjugated with Stevioside. Plants, 10(7), 1363.
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2

Antifungal Activity of Stevioside and Silymarin

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Stevioside (CAS 57817-89-7, 99%) was purchased from Wako Chemicals GmbH (Neuss, Germany). Powdered milk thistle extract (CAS 22888-70-6; 30% silybin, 98% HPLC) was purchased from KingHerbs Ltd. (Yongzhou, Hunan, China). Gallic acid (CAS 149-91-7; anhydrous, for synthesis), ethanol (CAS 64-17-5; ACS reagent grade), and Tween® 20 (CAS 9005-64-5) were supplied by Sigma-Aldrich/Merck KGaA (Darmstadt, Germany). Potato dextrose agar (PDA) and potato dextrose broth (PDB) were supplied by Becton, Dickinson & Company (Franklin Lakes, NJ, USA).
Fusarium culmorum strain CECT 20486 was supplied by the Spanish Type Culture Collection (CECT; Valencia, Spain).
Buzón-Durán L., Martín-Gil J., Ramos-Sánchez M.D., Pérez-Lebeña E., Marcos-Robles J.L., Fombellida-Villafruela Á, & Martín-Ramos P. (2020). Antifungal Activity against Fusarium culmorum of Stevioside, Silybum marianum Seed Extracts, and Their Conjugate Complexes. Antibiotics, 9(8), 440.
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