TIRF imaging and surface-generated structured illumination microscopy were performed on a Nikon Eclipse Ti inverted microscope (Nikon Instruments, Japan), equipped with a motorized TIRF illuminator, an ORCA-flash 4.0 sCMOS camera (Hamamatsu), a light emitting diode (LED)-based epifluorescence excitation source (SOLA, Lumencor), and a CO 2 and temperature control chamber (Okolab). In addition, a laser combiner (100 mW 405 nm, 60 mW 488nm, 50 mW 561 nm, and 100 mW 642nm solid-state lasers, Omicron Laserage) was coupled with a polarization-maintaining optical fiber. The objective used was a 60X N.A 1.49 Apo TIRF objective lens (Nikon Instruments).
N a 1.49 apo tirf objective lens
Manufactured by Nikon
Sourced in Italy
The 60X N.A 1.49 Apo TIRF objective lens is a high-performance optical component designed for various imaging and microscopy applications. It features a numerical aperture of 1.49 and a magnification factor of 60X, providing exceptional resolution and light-gathering capabilities. The lens is designed with an apochromatic (Apo) correction to minimize chromatic aberrations, ensuring accurate and precise imaging. Additionally, the lens is suitable for use in total internal reflection fluorescence (TIRF) microscopy, a technique that allows for the selective excitation of fluorophores near the coverslip surface.
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2 protocols using n a 1.49 apo tirf objective lens
1
Multi-Modal Microscopy for Cell Imaging
2
Live-cell TIRF Microscopy Imaging
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Live-cell Total Internal Reflection Fluorescence (TIRF) Microscopy TIRF imaging of live cells were performed using a Nikon Eclipse Ti inverted microscope (Nikon Instruments), equipped with a motorized TIRF illuminator, with a polarization-maintaining optical fiber-coupled laser combiner (100 mW 405 nm, 60 mW 488nm, 50 mW 561 nm, and 100 mW 642nm solid-state lasers, Omicron Laserage), a light emitting diode-based epifluorescence excitation source(-SOLA, Lumencor), an ORCA-flash 4.0 sCMOS camera (Hamamatsu), a 60X N.A 1.49 Apo TIRF objective lens (Nikon Instruments), and an Okolab stage-top chamber with CO 2 and temperature control (Okolab, Italy).
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