Lc ms ms tqd triple quad detector instrument

Efavirenz is administered as a once-daily dose given at night to ameliorate its potential neurological side-effects. In both of the children reported here, drug levels were collected during the day, before 12h00. Blood samples were collected in a 5 mL heparinised BD Vacutainer vial (BD Plymouth, UK). Specimens were stored at 2 -8˚C until analysed. Analysis was performed using liquid chromatographytandem mass spectrometry (LC-MS MS) at Ampath's laboratory in Johannesburg. An LC-MS MS TQD (triple quad detector) instrument from Waters in electrospray positive ionisation mode was used, employing an external standard from ChromSystems (Multilevel Plasma Calibrator Set Anti-HIV Drugs) for quantitation. Efavirenz is identified according to the molecular mass of its parent ion 316, as well as one daughter ion of molecular mass 244, and retention time.

EFV is administered as a once-daily dose given at night to limit side-effects, and blood samples for measurement of steady-state drug Corresponding author: M P K Hauptfleisch (marc.hauptfleisch@wits.ac.za) Acute ataxia in childhood is often caused by toxin ingestion. With the increasing number of paediatric patients on antiretroviral medication, we are seeing more side-effects of these drugs. We report two cases of efavirenz toxicity causing ataxia. November 2015, Vol. 105, No. 11 levels were collected from our patients during the day before 12h00. Samples were collected in a 5 mL heparinised BD Vacutainer vial (BD Plymouth PL6 7BP, UK) and stored at 2 -8 o C until analysed. Analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS MS) at Ampath Laboratory in Johannesburg. An LC-MS MS TQD (triple quad detector) instrument from Waters in electrospray positive ionisation mode was used, employing an external standard from ChromSystems (6PLUS1 Multilevel Plasma Calibrator Set Anti-HIV Drugs) for quantification. EFV is identified according to its molecular mass of parent ion 316 as well as one daughter ion of molecular mass 244 and retention time.