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Electric cell substrate impedance sensing ecis system

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The Electric Cell-Substrate Impedance Sensing (ECIS) system is a lab equipment used to measure the electrical impedance of cell cultures. It is designed to monitor the attachment, spreading, migration, and micromotion of cells in real-time.

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Lab products found in correlation

Forskolin, by MP Biomedicals (1 mentions) Rho kinase inhibitor y27632, by Cayman Chemical (1 mentions) Z vad fmk, by Merck Group (1 mentions) Necrostatin 1, by Merck Group (1 mentions) 3 methyladenine, by Merck Group (1 mentions) Ferrostatin 1, by Merck Group (1 mentions)

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ECIS) system (56 citations) Electric Cell-substrate Impedance Sensing (ECIS) (12 citations) Electric Cell Substrate Impedance Sensing system (9 citations)

5 protocols using electric cell substrate impedance sensing ecis system

1

Endothelial Barrier Integrity Measurement

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Endothelial barrier integrity was measured using an Electric Cell-substrate Impedance Sensing (ECIS) system (Applied BioPhysics, Troy, NY) as previously described [49 (link)]. Briefly, RMVECs were plated onto 8W10E+ arrays in normal culture medium and used when resistance reached ±900 Ohm, usually 24 h after seeding. Resistance was measured every 10 min for the duration of the experiments. Baseline resistance was measured for 1 h before addition of TAK-242, hemopexin, or PAK. TAK-242 and hemopexin were added 30 min before PAK.
Wagener B.M., Hu P.J., Oh J.Y., Evans C.A., Richter J.R., Honavar J., Brandon A.P., Creighton J., Stephens S.W., Morgan C., Dull R.O., Marques M.B., Kerby J.D., Pittet J.F, & Patel R.P. (2018). Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study. PLoS Medicine, 15(3), e1002522.
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2

Endothelial Barrier Regulation by H3Cit

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HUVECs were seeded onto 8W10E+ PET electrode arrays to be used with an Electric Cell-Substrate Impedance Sensing (ECIS) system (Applied Biophysics, Troy, NY). Transendothelial electrical resistance (TER) was continuously recorded over time as an indicator of cell-cell adhesive barrier function [22 ]. Cells were stimulated with human recombinant H3Cit (Item No. 17926; Cayman Chemical, Ann Arbor, MI) or vehicle control (0.1% BSA in PBS) with or without inhibitors. Representative tracings are presented normalized to baseline. Change in resistance was quantified by subtracting the lowest resistance value from the baseline resistance value of each ECIS well and averaging maximum change from baseline at each concentration. Inhibitors used in this study include Rho kinase inhibitor Y27632 (Cayman), Rho inhibitor Rhosin (Calbiochem), and MLCK inhibitor peptide 18 (Cayman). Barrier enhancing agent forskolin (MP Biomedicals) was used as a pharmacological therapeutic.
Meegan J.E., Yang X., Beard RS J.r., Jannaway M., Chatterjee V., Taylor-Clark T.E, & Yuan S.Y. (2018). Citrullinated Histone 3 Causes Endothelial Barrier Dysfunction. Biochemical and biophysical research communications, 503(3), 1498-1502.
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3

Electrical Impedance of A375 Cells

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Electrical impedance, resistance, and capacitance of A375 cells were measured using the Electric Cell-Substrate Impedance Sensing (ECIS) system (Applied Biophysics, Troy, NY). Cells were seeded in electrode-fitted wells at a sub-confluent density of 30,000 cells per 0.8 cm2, and electrode parameters were monitored at 32000 Hz over time. Medium change with different pHe values occurred at 24 hrs after cell seeding.
Yang O.C, & Loh S.H. (2019). Acidic Stress Triggers Sodium-Coupled Bicarbonate Transport and Promotes Survival in A375 Human Melanoma Cells. Scientific Reports, 9, 6858.
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4

Endothelial Barrier Integrity Measurement

Cited 1 time
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Endothelial barrier integrity was measured using an Electric Cell-substrate Impedance Sensing (ECIS) system (Applied Biophysics, Troy, NY) as previously described22 (link). Briefly, human umbilical vein endothelial cells (HUVECs) were plated onto 8W10E+ arrays in 10% FBS (fetal bovine serum) containing MCDB-131 medium and cultured until >95% confluency. HUVECs were incubated in 1% serum containing MCDB-131 for 2h before the experiment. Baseline resistance was measured 10min before addition of EVs (50μM total heme), or hemin chloride (5μM) and data collected every 2min for the duration of the experiment. In some experiments, EV solutions (10μL) were first purged with CO gas to ligate ferrous hemoglobin. Hemopexin (5μM) was added to test the role of free heme.
Dadusc G., Ogilvie J.P., Schulenberg P., Marvet U, & Miller R.J. (2001). Diffractive optics-based heterodyne-detected four-wave mixing signals of protein motion: From “protein quakes” to ligand escape for myoglobin. Proceedings of the National Academy of Sciences of the United States of America, 98(11), 6110-6115.
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5

Cell Barrier Function Modulation by Hemoglobin

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HLMVECs were seeded onto 8W10E+ PET electrode plates. The transendothelial electrical resistance (TER) was measured using an Electric Cell-Substrate Impedance Sensing (ECIS) system (Applied Biophysics, Inc). TER was used as an indicator of barrier function and was continuously measured over time. Cells were treated with vehicle control or native human cell-free hemoglobin (CFH; Cell Sciences Hb3+, CSI19668B) that was dissolved in PBS and sterile filtered, with or without a one-hour pretreatment of different cell death inhibitors, including an apoptosis inhibitor (Z-VAD-FMK, Sigma V116; 0.1 μM), necrosis inhibitor (IM-54, Millipore 480060; 5 μM), necroptosis inhibitor (necrostatin-1, Sigma N9037; 5 μM), ferroptosis inhibitor (ferrostatin-1, Sigma SML0583; 1 μM), and autophagy inhibitor (3-methyladenine, Sigma M9281; 0.2 mM). The resistance at a given time was subtracted from the baseline resistance (T=0) of each well and averaged to determine change in TER (TER Δ).
Tomasek T., Ware L.B., Bastarache J.A, & Meegan J.E. (2021). Cell-free hemoglobin-mediated human lung microvascular endothelial barrier dysfunction is not mediated by cell death. Biochemical and biophysical research communications, 556, 199-206.
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