Distearoyl phosphatidylcholine dspc

Manufactured by Avanti Polar Lipids

Sourced in United States

Distearoyl phosphatidylcholine (DSPC) is a naturally occurring phospholipid used in various laboratory applications. It is a common component of biological membranes and has a well-defined molecular structure. DSPC is often utilized in research and development related to liposome formulations, membrane models, and drug delivery systems.

Automatically generated - may contain errors

Lab products found in correlation

Perfluorohexane pfh, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Cytiva (1 mentions) Oleic acid, by Merck Group (1 mentions) 1 octadecene, by Merck Group (1 mentions) Milli q plus system, by Merck Group (1 mentions) Perfluorohexane, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Ebm 2 media, by Lonza (1 mentions) Triethylamine, by Thermo Fisher Scientific (1 mentions) Cholesterol, by Tokyo Chemical Industry (1 mentions) Milli q gradient system, by Merck Group (1 mentions) Cell culture dishes and plates, by Corning (1 mentions)

Close spelling variants of this product

Phosphatidylcholine (82 citations) 1,2-distearoyl-sn-glycero-3-phosphatidylcholine (DSPC) (5 citations) Powders of 1,2-distearoyl-sn-glycero-3-phosphatidylcholine(DSPC (2 citations)

4 protocols using distearoyl phosphatidylcholine dspc

Lipid-Based Nanoparticle Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Distearoyl phosphatidylcholine (DSPC) was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL) and distearoyl phosphatidylethanolamine-methyl poly(ethylene glycol) MW5000 (mPEG-DSPE 5k) was purchased from Laysan Bio, Inc. (Arab, AL). Dulbecco’s phosphate buffered saline (DPBS) was purchased from Hyclone Laboratories Inc. (Logan, UT). The lipophilic carbocyanine dye, DiI, was purchased from Biotium, Inc. (Hayward, CA). Perfluorohexane (PFH) was purchased from Sigma-Aldrich (St. Louis, MO).

Schutt C.E., Ibsen S.D., Benchimol M.J., Hsu M.J, & Esener S.C. (2014). Manipulating Nanoscale Features on the Surface of Dye-Loaded Microbubbles to Increase their Ultrasound-Modulated Fluorescence Output. Small (Weinheim an der Bergstrasse, Germany), 10(16), 3316-3324.

+ Open protocol

+ Expand

Synthesis of b-NaYF4:Yb,Er Nanoparticles

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All of the chemicals used were of analytical grade and were used without
further purification. Rare earth chlorides, oleic acid, 1-octadecene was were
purchased from Sigma-Aldrich. Phospholipids, distearoylphosphatidylcholine
(DSPC), were purchased from Avanti Polar Lipids. The wild-type apo-azurin was
expressed and purified as previously reported.^{12a (link)} b-NaYF₄: Yb, Er were
synthesized according to the previously reported method using rare earth
chlorides as precursor and oleic acid as stabilizing agents.^{14 (link)}

Liu C., Yu Y., Chen D., Zhao J., Yu Y., Li L, & Lu Y. (2019). Cupredoxin engineered upconversion nanoparticles for ratiometric luminescence sensing of Cu2+. Nanoscale Advances, 1(7), 2580-2585.

+ Open protocol

+ Expand

Nanoparticle Production and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Perfluorohexane was purchased from Sigma-Aldrich (St. Louis, MO, USA). Distearoyl phosphatidylcholine (DSPC), was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). 2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[maleimide (polyethylene glycol)-3400] (DSPE-PEG3400-Malemide) was purchased from Laysan Bio, Inc. (Arab, AL, USA). DiO was purchased from Biotium, Inc. (Hayward, CA, USA). The trypsin (.25% T / 2.21 mM EDTA) was purchased from Mediatech Inc. (Manassas, VA, USA). Human Umbilical Vein Endothelial Cells (HUVEC) and 4T1 breast carcinoma cells were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). EBM-2 media was purchased from Lonza Inc (Basel, Switzerland). The cRGD was purchased from Anaspec Incorporated (Fremont, CA, USA). The penicillin-streptomycin used in the EBM-2 media was purchased from Gibco (Invitrogen, Grand Island, NY, USA). The fetal bovine serum used in the EBM-2 media solution was purchased from Hyclone (Logan, UT, USA). The PBS was purchased from Hyclone Laboratories Inc. (Logan, UT, USA). All water was purified using the Milli-Q Plus System (Millipore Corporation, Bedford, USA). Purified rat anti-mouse CD326 antibody was obtained from Bio Legend (San Diego, CA, USA).

Ibsen S., Shi G., Schutt C., Shi L., Suico K.D., Benchimol M., Serra V., Simberg D., Berns M, & Esener S. (2014). The Behavior of Lipid Debris Left on Cell Surfaces from Microbubble Based Ultrasound Molecular Imaging. Ultrasonics, 54(8), 2090-2098.

+ Open protocol

+ Expand

Nanoparticle-based Immunotherapy Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gadolinium acetate hydrate and skeletal muscle Mb were purchased from Sigma–Aldrich (USA). Triethylamine was purchased from Alfa Aesar (USA). Distearoylphosphatidylcholine (DSPC) and DSPC-polyethylene glycol 2000 (DSPE-mPEG2000) were purchased from Avanti Polar Lipids (USA). Cholesterol was purchased from Tokyo Chemical Industry (Japan). Cell culture dishes and plates were purchased from Corning (USA). Mouse IFN-γ ELISA Kit (Catalog No. CZM10-96) was purchased from Beijing CHENG ZHI KE WEI Biotechnology Co., Ltd. (China). Mouse IL-1β ELISA Kit (Catalog No. EMC001bQT) was purchased from NeoBioscience Technology Co., Ltd. (China). Ultrapure water was obtained using a Millipore Milli-Q Gradient System (USA). Rabbit anti-γ-H2A.X (phospho S139) antibody (Catalog No. ab81299, Clone: EP854(2)Y, 1: 250) was purchased from Abcam (USA). FITC-conjugated goat anti-rabbit IgG H&L antibody (Catalog No. ab6717, 1: 1000) was purchased from Abcam (USA). APC anti-mouse CD3 antibody (Catalog No. 100312, Clone: 145-2C11, 1: 100), FITC anti-mouse CD8 antibody (Catalog No. 100706, Clone: 53-6.7, 1: 100), PE/Cyanine7 anti-mouse IFN-γ antibody (Catalog No. 505826, Clone: XMG1.2, 1: 100), PE anti-mouse/human CD44 antibody (Catalog No. 103008, Clone: IM7, 1: 100), and PE/Cyanine7 anti-mouse CD62L antibody (Catalog No. 104418, Clone: MEL-14, 1: 100) were purchased from BioLegend (USA).

Ma X., Liang X., Yao M., Gao Y., Luo Q., Li X., Yu Y., Sun Y., Cheng M.H., Chen J., Zheng G., Shi J, & Wang F. (2023). Myoglobin-loaded gadolinium nanotexaphyrins for oxygen synergy and imaging-guided radiosensitization therapy. Nature Communications, 14, 6187.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!