Human pharyngeal SCC cell line FaDu (ATCC, HTB-43) was grown in Advanced Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Thermo Fisher, MA, USA) supplemented with 5% fetal bovine serum (FBS, Gibco, Thermo Fisher), 10 mM L-glutamine (GlutaMAX, Gibco), penicillin (100 U/mL) (Grünenthal, Germany) and gentamicin (50 mg/mL) (Krka, Slovenia). Cells were routinely subcultured twice a week and incubated in a humidified atmosphere at 37 °C and 5% CO2.
The HPV-positive 2A3 cell line, a kind gift from Prof. Dadachova was established by transfection of FaDu cells with HPV16 oncogenes E6 and E7 [12 (link)]. 2A3 cells were grown in Advanced DMEM supplemented with 5% fetal bovine serum, 10 mM L-glutamine, penicillin (100 U/mL), gentamicin (50 mg/mL) and 1 mg/mL G418 disulfate salt solution (Sigma-Aldrich, MO, USA).
Authentication of FaDu, FaDu-RR, and 2A3 cells by short tandem repeats profiling was performed using CellCheck 16 – human (IDEXX BioAnalytics, Germany) for authentication of FaDu, and FaDu-RR cells (Additional file1 : Final report of laboratory examination) and CellCheck 9 – human (IDEXX BioAnalytics) for 2A3 cells (Additional file 2 : Final report of laboratory examination). The genetic profile of the cell lines used for the study was identical to the publically available genetic profile of these cell lines.
The HPV-positive 2A3 cell line, a kind gift from Prof. Dadachova was established by transfection of FaDu cells with HPV16 oncogenes E6 and E7 [12 (link)]. 2A3 cells were grown in Advanced DMEM supplemented with 5% fetal bovine serum, 10 mM L-glutamine, penicillin (100 U/mL), gentamicin (50 mg/mL) and 1 mg/mL G418 disulfate salt solution (Sigma-Aldrich, MO, USA).
Authentication of FaDu, FaDu-RR, and 2A3 cells by short tandem repeats profiling was performed using CellCheck 16 – human (IDEXX BioAnalytics, Germany) for authentication of FaDu, and FaDu-RR cells (Additional file