129 sv mice

All animal experiments were reviewed and approved by the Animal Studies Committee of Yokohama City University, which is in compliance with the ARRIVE guidelines. Efforts were made to minimize the number of animals used and ensure minimal suffering. Male 129/Sv mice were purchased from Japan SLC (Shizuoka, Japan). They were housed in a controlled environment on a 12-h light/dark cycle at a temperature of 25 °C and were allowed free access to an ND (24 % of total calories from protein, 59% carbohydrate, and 17% fat; Clea, Osaka, Japan) or HPD (45% protein, 38% carbohydrate and 17% fat; Clea, Osaka, Japan) and water.

All animal care and experimental procedures were performed in accordance with the guidelines of the Committee for Animal Experimentation at Kirin Company. These studies were approved by the Committee for Animal Experimentation at Kirin Company. Mice were kept in a room maintained on a 12:12-h light-dark cycle at a temperature of 23 ± 1 °C. Mice for BM-DCs assays were provided with a standard mouse chow (CE-2; CLEA Japan, Tokyo, Japan) and mice for the in vivo study were provided with AIN-93G (Oriental Yeast, Tokyo, Japan). BALB/c, C57BL/6 J and C57 BL/6 J Tlr4^−/−, Tlr9^−/− and Myd88^−/− mice were obtained from Charles River Laboratories Japan (Kanagawa, Japan). 129/Sv mice were obtained from Japan SLC, Inc. (Shizuoka, Japan).