The size-exclusion chromatography system was a Waters Alliance 2695 Separations Module fitted with a Waters 2996 Photodiode Array Detector (Waters Corporation, Milford, MA, USA). Instrument operation and data acquisition and manipulation were carried out with Waters Empower 2 Chromatography Manager (Waters Corporation). A YMC-Pack Diol-200 column (Product# DL20S05-5008WT, YMC America, Inc., Allentown, PA, USA) with internal dimensions of 500×8.0 mm was used at a flow rate of 0.8 ml/min. The mobile phase consisted of 0.1 M sodium phosphate, 0.15 M sodium chloride (pH 7.0) and peaks were detected at a wavelength of 214 nm.
Waters alliance 2695 separations module
Manufactured by Waters Corporation
Sourced in United States
The Waters Alliance 2695 Separations Module is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative separations. It features automated sample handling, a built-in solvent management system, and precise temperature control to ensure reliable and reproducible results. The core function of the Alliance 2695 is to separate, identify, and quantify components within a sample.
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Lab products found in correlation
Waters 2996 photodiode array detector, by Waters Corporation (1 mentions)
Waters empower 2 chromatography manager, by Waters Corporation (1 mentions)
Waters 2487 dual λ absorbance detector, by Waters Corporation (1 mentions)
Phenol, by Merck Group (1 mentions)
Micro syringe, by CAMAG (1 mentions)
Linomat autosampler, by CAMAG (1 mentions)
Infra red spectrometer, by Shimadzu (1 mentions)
4 protocols using waters alliance 2695 separations module
1
Size-Exclusion Chromatography Protocol
2
Quantification of Asarones in SCP Extract
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The standards including α-asarone (purity: 100%, National Institutes for Food and Drug Control, China) and β-asarone (purity: 95.52%, ChromaDex, USA) were accurately weighted and dissolved in pure methanol as the standard solutions. One and a half grams of the SCP extract powder was dissolved in 25 mL methanol, and the solution was centrifuged at 9000 rpm at 4 °C for 10 min. The supernatant was collected as the test solution. Subsequently, High performance liquid chromatography (HPLC) analytical procedures were performed, as described previously [39 (link)]. Briefly, twenty microliters of the standard or test solution was injected into a Waters HPLC system (Waters Alliance 2695 Separations Module, Waters Corp.). The mobile phase consisted of water (A) and acetonitrile (B). In gradient elution, the percentage of mobile phase A was decreased from 75 to 25% for 50 min, whereas the amount of mobile phase B was increased from 25 to 75%. The flow rate was set at 1.0 mL per min, and the total run time was 50 min. Absorbance was measured at 257 nm.
3
Photocatalytic Degradation of Phenol
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For evaluation of the photocatalytic performances of MgAC-Fe3O4/TiO2 hybrid nanocomposites, 0.1 g of MgAC-Fe3O4/TiO2 hybrid nanocomposites was loaded into a petri dish of 100 mL of phenol at 5 ppm (≥99%; Sigma-Aldrich, St. Louis, MO, USA). After obtainment of equilibrium adsorption, a 365 nm wavelength UV light source (~610 µW/cm2) was turned on. Interval samples were withdrawn after each 20 min, and the remaining organic compounds were detected by high-performance liquid chromatography (for phenol; HPLC, Waters Alliance 2695 Separations Module equipped with Waters 2487 Dual λ Absorbance Detector; Waters, Milford, MA, USA) under the mobile phase of water and acetonitrile in a ratio of 40:60 (v/v) with a flow rate of 1 mL/min59 . For detection of phenol, UV absorption was performed at 270 nm.
The recycle usage experiments were conducted to check the stability of materials. After photocatalyst materials were separated from degraded solution, they were washed with DI water and ethanol, then dried in the oven at 60 °C for 12 hours. Then the materials is ready for another photocatalytic experiments. This method was repeated for 5 times53 .
The recycle usage experiments were conducted to check the stability of materials. After photocatalyst materials were separated from degraded solution, they were washed with DI water and ethanol, then dried in the oven at 60 °C for 12 hours. Then the materials is ready for another photocatalytic experiments. This method was repeated for 5 times53 .
4
Analytical Characterization of Organic Compounds
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The thin-layer chromatography (TLC) system consists of 100 μL Camag micro syringe (Muttenz, Switzerland) as well as Camag Linomat autosampler (Camag, Switzerland). TLC was performed utilizing 0.25 mm thickness TLC plates pre-coated with silica gel G.F254 20X20 cm (Merck, Darmstadt, Germany), winCATS software, and a short wavelength (254 nm) ultraviolet (UV) lamp (USA).
The HPLC system consists of Waters Alliance 2695 Separations Module using inline vacuum degassing with different flow rates, an autosampler, and programmable temperature control. A heated column compartment provides temperatures from 5 degrees above ambient to 65 °C. The wavelength range of model 2996 photodiode array detector is 190–800 nm. Stationary phase was Kromasil® MS C18 column (150 mm × 4.6 mm, 5 µm) (Nouryon, Bohus, Sweden).Whatman syringe filter (Fisher, Nederland) was utilized. For IR and mass spectrometric analysis, an infrared (IR) spectrometer (Shimadzu, Japan) and an Ultra-performance liquid chromatography-mass spectrometer (LC/MS/MS) (Waters, Milford, USA) were used. Design-Expert software was used for data analysis (version 12) (Godward St NE, Minneapolis). Hot plate and pH meter were used (Jenway, Stone, UK).
The HPLC system consists of Waters Alliance 2695 Separations Module using inline vacuum degassing with different flow rates, an autosampler, and programmable temperature control. A heated column compartment provides temperatures from 5 degrees above ambient to 65 °C. The wavelength range of model 2996 photodiode array detector is 190–800 nm. Stationary phase was Kromasil® MS C18 column (150 mm × 4.6 mm, 5 µm) (Nouryon, Bohus, Sweden).Whatman syringe filter (Fisher, Nederland) was utilized. For IR and mass spectrometric analysis, an infrared (IR) spectrometer (Shimadzu, Japan) and an Ultra-performance liquid chromatography-mass spectrometer (LC/MS/MS) (Waters, Milford, USA) were used. Design-Expert software was used for data analysis (version 12) (Godward St NE, Minneapolis). Hot plate and pH meter were used (Jenway, Stone, UK).
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