Hrp anti myc tag antibody

CUT&Tag analysis was performed as described in a previous study [43 (link)]. The primary antibody was the HRP Anti-Myc tag antibody (at 1:50 dilution; Abcam plc, ab62928, Cambridge, UK), with more details provided in the Supplementary Information 1. The raw sequencing data can be requested from the authors.

We used 2 g young inflorescence tissues from each genotype for Co-immunoprecipitation (Co-IP). Anti-HA tag antibodies (ABclonal) were coupled to magnetic beads by Dynabeads antibody coupling kit (Invitrogen) following the description in the manual. We used 1.5 mg antibody-coupled beads for each sample in the Co-IP experiment. In the case of ASF1s-H3s, we used Co-IP anti-FLAG M2 magnetic beads (SIGMA). Described of the detailed procedure is in our previous publication^{62 (link)}. We used HRP anti-Myc tag antibody (Abcam) with 1:10000 dilution to perform western blots.

The GFP‐ and GFP‐FocSso1‐expressing strains were cultured in liquid CM with constant agitation at 28°C for 2 days. The resulting mycelia were filtered, washed with sterile distilled water and ground into powder in liguid nitrogen. The powder was suspended in lysis buffer containing a cocktail of protease inhibitor for 30 min. The suspension was then centrifuged and the supernatant was collected. About 30 μL of GFP‐Trap_A beads (ChromoTek Inc., Planegg‐Martinsried) was added to the supernatant, and the mixture was incubated for 4 h at 4°C. The proteins bound to the beads were then eluted by heating at 100°C for 10 min. Subsequently, mass spectrometry analysis was performed following a previously established method (Zheng et al., 2018 (link)). The protein samples for Co‐IP were extracted using the same method as described previously. Following boiling, the protein samples were separated using 10% SDS‐PAGE and subsequently analysed using western blotting. The analysis involved the use of anti‐GFP antibody (GFPTag (7G9) mouse mAb; Abmart), goat anti‐mouse IgG horseradish peroxidase (HRP) (Abmart), and anti‐Myc antibody (HRP anti‐Myc tag antibody; Abcam).