Hesperidin hes

Human Detroit 562 cells were purchased from the European Collection of Authenticated Cell Cultures (catalogue no. 87042205, European Collection of Authenticated Cell Cultures, Porton Down, Salisbury, England). Eagle's minimum essential medium (EMEM), fetal bovine serum (FBS) and penicillin-streptomycin cocktails were purchased from Thermo Scientific (Rockford, IL, USA). Bioflavonoids compounds rutin (Rut), quercetin (Que) and hesperidin (Hes) were obtained from Sigma-Aldrich (St. Louis, MO, USA) and the Muse Annexin & Dead Cell kit and the Muse Count and Viability kit is manufactured by Millipore (Darmstadt, Germany).
Pharynx cancer cell line Detroit 562 was grown according to the manufacturer's specification using EMEM supplemented with 10% FBS. The cells were cultured in bottles of 75 cm 2 (PAA) with the addition of antibiotics: 100 IU/mL penicillin, 100 µL/mL streptomycin and 0.25 µL/mL amphotericin B. Cells were cultured in a CO 2 incubator at 37 • C and in a saturated steam atmosphere with 5% carbon dioxide (CO 2 ).