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Percpcy5.5 labeled anti mouse ly 6c

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PerCPcy5.5-labeled anti-mouse Ly-6C is a fluorescently-labeled antibody used for the detection and identification of Ly-6C, a cell surface marker, in mouse cells. It can be used in flow cytometry applications.

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Lab products found in correlation

Pe labeled anti mouse gr 1, by Thermo Fisher Scientific (2 mentions) Fitc labeled anti mouse ly 6g, by Thermo Fisher Scientific (2 mentions) Apc labeled anti mouse f4 80, by Thermo Fisher Scientific (2 mentions) Pe labeled anti mouse pdl 1, by Thermo Fisher Scientific (2 mentions) Pecy 7 labeled anti mouse cd11c, by Thermo Fisher Scientific (2 mentions) Pe labeled anti mouse cd115, by Thermo Fisher Scientific (2 mentions) Apccy 7 labeled anti mouse cd11b, by BD (2 mentions) Pe labeled anti mouse cd124, by BD (2 mentions) Pe labeled anti mouse inos, by Santa Cruz Biotechnology (2 mentions)

2 protocols using percpcy5.5 labeled anti mouse ly 6c

1

Phenotyping of In Vitro Differentiated BM-MDRCs

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The in vitro differentiated BM-MDRC were prepared for flow cytometry by first incubating in FACS staining buffer (PBS + 3% FBS) containing 2.0 μg/ml of the mAb 2.4G2 (BD Pharmingen, Franklin Lakes, NJ) at 4°C for 30 minutes. These cells were then stained with several fluorochrome conjugated anti-mouse monoclonal antibodies for cellular phenotyping as follows: PE- labeled anti-mouse Gr-1 (clone: RB6-8C5), PerCPcy5.5-labeled anti-mouse Ly-6C (clone: HK1.4), FITC-labeled anti-mouse Ly-6G (clone: 1A8), APC-labeled anti-mouse F4/80 (clone: BM8), PE- labeled anti-mouse PDL-1 (clone: M1H5), PECy-7-labeled anti-mouse CD11c (clone: N418), PE-labeled anti-mouse CD115 (clone: AFS98) (all these antibodies were obtained from eBiosciences, San Diego CA); APCCy-7 labeled anti-mouse CD11b (clone: M1/70) and PE-labeled anti-mouse CD124 (clone: mIL4-R-M1) from BD Biosciences, San Jose, CA; and PE-labeled anti-mouse iNOS (Santa Cruz Biotechnologies, Dallas, TX).
Wang Y., Jin T.H., Farhana A., Freeman J., Estell K., Zmijewski J., Gaggar A., Thannickal V.J., Schwiebert L.M., Steyn A.J, & Deshane J.S. (2014). Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. Laboratory investigation; a journal of technical methods and pathology, 94(12), 1312-1325.
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2

Phenotyping of In Vitro Differentiated BM-MDRCs

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The in vitro differentiated BM-MDRC were prepared for flow cytometry by first incubating in FACS staining buffer (PBS + 3% FBS) containing 2.0 μg/ml of the mAb 2.4G2 (BD Pharmingen, Franklin Lakes, NJ) at 4°C for 30 minutes. These cells were then stained with several fluorochrome conjugated anti-mouse monoclonal antibodies for cellular phenotyping as follows: PE- labeled anti-mouse Gr-1 (clone: RB6-8C5), PerCPcy5.5-labeled anti-mouse Ly-6C (clone: HK1.4), FITC-labeled anti-mouse Ly-6G (clone: 1A8), APC-labeled anti-mouse F4/80 (clone: BM8), PE- labeled anti-mouse PDL-1 (clone: M1H5), PECy-7-labeled anti-mouse CD11c (clone: N418), PE-labeled anti-mouse CD115 (clone: AFS98) (all these antibodies were obtained from eBiosciences, San Diego CA); APCCy-7 labeled anti-mouse CD11b (clone: M1/70) and PE-labeled anti-mouse CD124 (clone: mIL4-R-M1) from BD Biosciences, San Jose, CA; and PE-labeled anti-mouse iNOS (Santa Cruz Biotechnologies, Dallas, TX).
Wang Y., Jin T.H., Farhana A., Freeman J., Estell K., Zmijewski J., Gaggar A., Thannickal V.J., Schwiebert L.M., Steyn A.J, & Deshane J.S. (2014). Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. Laboratory investigation; a journal of technical methods and pathology, 94(12), 1312-1325.
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