The reflectance spectra of four healthy male and female volunteers skin were recorded in the 450–1750 nm range with 1 nm resolution with Cary 5000 Varian spectrophotometer (a high-performance UV–Vis and NIR model). Double beam and a 0°/d measurement geometry has been utilized in our measurements with internal DRA (Diffuse Reflectance Accessory) that includes an integrating sphere of 110 mm diameter. The volunteers did not weare any cosmetic product in the study area (dorsal side of wrist) for at least 24 h and washed their hands 2 h before the measurements were taken. Informed consent has been obtained from all subjects prior to participation in the study with collected data appropriately encoded and subsequently processed. Initially, we captured reflectance spectra of the dry and wet skin of the dorsal surface of the wrists of the left hand. The reflectance spectra were repeatedly acquired at the medial region of dorsal surface of left hand for 3 points in each site located at 2 cm from each other.
Cary 5000 varian spectrophotometer
Manufactured by Agilent Technologies
The Cary 5000 Varian spectrophotometer is a versatile and high-performance laboratory instrument designed for precise and accurate spectroscopic measurements. It is capable of performing a wide range of analyses, including UV-Vis-NIR absorption, transmission, and reflectance measurements across a broad wavelength range.
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4 protocols using cary 5000 varian spectrophotometer
1
Skin Reflectance Spectra of Healthy Volunteers
2
Diffuse Reflectance NIR Spectroscopy of TiO2 Nanoparticles
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Diffuse reflectance (DR) NIR spectra were recorded with a Cary 5000 Varian spectrophotometer equipped with an integrating sphere with an inner coating of Spectralon®. This material was used also as reference. For the collection of spectra, thick self-supported pellets of TiO2 nanoparticles were placed in a cell with an optical quartz window designed to carry out the measurements in controlled atmosphere, by connection to a conventional vacuum line. Before dosing H2O, the TiO2 samples were activated with the same procedure employed for Mid-IR spectroscopy.
3
Elemental and Spectroscopic Characterization
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Elemental analyses for carbon, hydrogen and nitrogen were carried out with a Perkin Elmer 2400 analyzer. FT IR spectra in the range 4000–400 cm−1 and 600–50 cm−1 were collected with a Perkin Elmer FTIR/FIR Spectrum 4000 spectrophotometer by attenuated total reflectance (ATR). NMR spectra were obtained at room temperature on a 400 MHz VNMRS Varian with Broad Band Switchable probe of two channel ratio frequency (1H/19F) (31P/15N) spectrophotometer of 9.4 T. Electronic spectra were measured over the range 40,000–5000 cm−1 by the diffuse reflectance method on a Cary-5000 Varian spectrophotometer at room temperature. Spectra of solution spectra in sodium cacodylate, 3-(N-morpholino)-propanesulfonic acid) (MOPS) and phosphate-buffered saline (PBS) were collected in the range of 1600–250 nm. Magnetic susceptibility measurements at room temperature of powdered samples were obtained on a Sherwood Scientific MK1 magnetic susceptibility balance, using the Gouy method.
4
Characterization of Plasma-Treated MIL-125(Ti)-NH2 MOF
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Powder X-ray diffractograms (PXRD) of plasma-treated MIL-125(Ti)-NH2 samples were recorded in a Philips XPert diffractometer (40 kV and 45 mA) using Ni filtered Cu Kα radiation. Diffuse reflectance UV-visible spectra of the solids were recorded in a Cary 5000 Varian spectrophotometer with an integrating sphere where the sample as compressed powder was placed in a sample holder. X-ray photoelectron spectra (XPS) were recorded using a SPECS spectrometer equipped with a MCD-9 detector using a monochromatic Al (Kα= 1486.6 eV) X-ray source. Spectra deconvolution was performed after Shirley subtraction of background with the CASA software using the C 1s peak at 284.4 eV as binding energy reference. Fourier-transform infrared (FTIR) spectra were recorded on compressed powders using a Bruker spectrophotometer in an attenuated total reflectance (ATR) cell. The morphology of the MOF samples was characterized using a scanning electron microscope (SEM, Zeiss instrument, AURIGA Compact). MOF particle size distribution was estimated for each sample by measuring more than 300 particles.
Isothermal N 2 adsorption measurements of the MIL-125(Ti) solids were carried out using an ASAP 2010 Micromeritics station.
Isothermal N 2 adsorption measurements of the MIL-125(Ti) solids were carried out using an ASAP 2010 Micromeritics station.
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