Qiasymphony dsp virus pathogen midi kit

The clinical and laboratory data of the patients were collected in accordance with the structured report released by the “Società Italiana di Radiologia Medica e Interventistica (SIRM)” [17 ]. Comorbidities included in the analysis were: presence of diabetes, obesity (i.e. BMI > 30), hypertension, smoke history, ACEi/Sartan or FANS therapy. As for clinical data: fever, cough, rhinitis, dyspnea, anosmia, dysgeusia, pharyngodynia, myalgias, asthenia, conjunctivitis, headache, nausea, vomit and diarrhea. Regarding laboratory data: white blood cells (WBC) count (neutrophils, lymphocytes), procalcitonin (PCT), C-reactive protein (CRP), lactate dehydrogenase (LDH), hepatic enzymes, creatine kinase (CK), blood pH and arterial partial pressure of oxygen (PaO₂) and carbon dioxide (PaCO₂) and fraction of inspired oxygen (FiO₂). Moreover, we calculated the neutrophil–to-lymphocyte ratio (NLR), the alveolar–arterial gradient (A–a gradient) and the PaO₂/FiO₂ ratio (P/F). The RT-PCR protocol included extraction with QIAsymphony^® DSP Virus/Pathogen Midi Kit and amplification with Seegene AllplexTM 2019-nCoV Assay (target genes E, N, RdRP).

The demographic data, comorbidities, clinical and laboratory data of the patients were collected in accordance with the structured report released by the “Società Italiana di Radiologia Medica e Interventistica (SIRM)” [13 ]. As for comorbidities, variables included in the analysis were: presence of cardiac disease, diabetes, obesity, hypertension, smoke history, ACEi/Sartan or FANS therapy. As for clinical data: Fever, Cough, Rhinitis, Dyspnea, Pharyngodynia, Myalgias, Asthenia, Conjunctivitis, Headache, Nausea, Vomit and Diarrhea.
Regarding Laboratory data: white blood cells (WBC) count, C-reactive protein (CRP), Lactate dehydrogenase (LDH), hepatic enzymes, creatin kinase (CK), blood’s pH and arterial partial pressure of carbon dioxide (PaCO2). RT-PCR protocol: extraction with QIAsymphony^® DSP Virus/Pathogen Midi Kit, amplification with Seegene AllplexTM 2019-nCoV Assay (target genes E, N, RdRP).