Malachite green oxalate

Manufactured by Merck Group

Sourced in United States

Malachite green oxalate is a synthetic dye compound commonly used as a laboratory reagent. It serves as a colorimetric indicator, primarily utilized in various analytical and biological applications.

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Lab products found in correlation

Splintr ligase, by New England Biolabs (2 mentions) Bromothymol blue, by Merck Group (1 mentions) Fuchsine, by Merck Group (1 mentions) Coomassie brilliant blue g 250, by Merck Group (1 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions) Recombinant rnase inhibitor, by Takara Bio (1 mentions) Ribonucleotide solution mix, by New England Biolabs (1 mentions) Dfhbi 1t, by Bio-Techne (1 mentions) T7 rna polymerase, by New England Biolabs (1 mentions) Tris hcl, by Bioneer (1 mentions) Crystal violet, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Tris hcl ph7, by Thermo Fisher Scientific (1 mentions) Murine rnase inhibitor, by Vazyme (1 mentions) T4 dna ligase, by New England Biolabs (1 mentions) Specord 205, by Analytik Jena (1 mentions) Icap 6000 series icp oes, by Thermo Fisher Scientific (1 mentions) Dicetyl phosphate, by Merck Group (1 mentions) Phosphatidylserine, by Merck Group (1 mentions) Perchloric acid, by Fujifilm (1 mentions)

Close spelling variants of this product

Malachite green oxalate salt Malachite green (MLG) oxalate salt Malachite green

7 protocols using malachite green oxalate

Characterization of Membrane Filters

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Remarks on the intact PTFE membrane, porous PTFE membrane, filter paper, nitrocellulose filter (NCF), polycarbonate membrane (PCM), and JHWP04700 are listed in Table 1. The contents of a conventional material transfer agreement restricted the disclosure of information on iPTFE and pPTFE.
Reagents: amide black 10 B (AB), bromothymol blue (BTB), Coomassie brilliant blue G250 (CBB), fluorescein (FL), fuchsine (FC), LDC, malachite green oxalate (MG), methylene blue (MB), methyl orange (MO), rhodamine 6G (R6G), Sudan III (SD) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Wako Pure Chemical Industries (Osaka, Japan). All other reagents were of the highest commercially available grade.

Mitsuya K., Goto S., Otsuka Y., Kawano Y, & Hanawa T. (2022). Saturated adsorption of lidocaine and coal tar dyes onto porous polytetrafluoroethylene. RSC Advances, 12(4), 1914-1921.

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In Vitro Transcription and RNA Detection

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SplintR ligase, T7 RNA polymerase, extreme thermostable single-stranded DNA binding protein (ET-SSB), DNase I (RNase-free) and ribonucleotide solution mix were obtained from New England Biolabs. Recombinant RNase inhibitor was obtained from Takara. Malachite green oxalate was purchased from Sigma–Aldrich. DFHBI-1T was purchased from Tocris Bioscience. Dithiothreitol was acquired from Thermo Fisher Scientific. Tris-HCl, potassium chloride, sodium chloride, magnesium chloride and 5′ phosphate modified oligonucleotides were obtained from Bioneer. Full-length probe oligonucleotides were synthesized by Integrated DNA Technologies. Oligonucleotides other than these were synthesized by Cosmogenetech.

Woo C.H., Jang S., Shin G., Jung G.Y, & Lee J.W. (2020). Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription. Nature Biomedical Engineering, 4(12), 1168-1179.

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TiO2 Nanoparticles Synthesis and Dye Removal

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TiO₂ nanoparticles (rutile phase, 99.9%, 30 nm) were purchased from US Research Nanomaterials Co. (Houston, TX, USA) Malachite green oxalate and crystal violet were obtained from Sigma Aldrich (St. Louis, MO, USA). H₂O₂ (30–35%) and NaOH were supplied from Mojallali Co. Ltd. (Tehran, Iran). HCl (37%) was purchased from Merck (Darmstadt, Germany). All solutions were prepared with distilled water.

Khaledian H.R., Zolfaghari P., Elhami V., Aghbolaghy M., Khorram S., Karimi A, & Khataee A. (2019). Modification of Immobilized Titanium Dioxide Nanostructures by Argon Plasma for Photocatalytic Removal of Organic Dyes. Molecules, 24(3), 383.

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Oligonucleotide-based protocol synthesis

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All the oligonucleotide probes, blocker, and target sequences we used were synthesized at GENEWIZ Inc. (Suzhou, China) and these sequences were listed in Supplementary Tables S1 and S2. Splint R ligase and T4 DNA ligase were obtained from New England Biolabs (Ipswich, MA, USA). Murine RNase Inhibitor was acquired from Vazyme Biotech (Nanjing, China). Malachite green oxalate was purchased from Sigma–Aldrich (Darmstadt, Germany). T7 RNA polymerase was purchased from TaKaRa Biomedical Technology Co., Ltd (Beijing, China). NTPs was obtained from Sangon Biotech. (Shanghai, China). Tris-HCL (pH 7.5) was acquired from Thermo Fisher Scientific (Waltham, Massachusetts, USA). And magnesium chloride solution was obtained from J&K Scientific Ltd. (Beijing, China). ATP was purchased from MDBio, Inc (Taiwan, China).

Gao Y., Wang T., Li J., Wei Y, & Qi H. (2022). Toehold-controlled ligation and transcription for accurate COVID-19 genotyping. Analytical Biochemistry, 654, 114803.

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Soil and Plant Phosphorus Extraction

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For extraction of P_labile about 100 mg of dried, milled soil was used. The samples were suspended in 10 ml Bray-1 solution (1 M NH₄F, 0.5 M HCl) and shaken for 5 min at 180 rpm. The samples were afterwards filtered using phosphate free filter paper (MN 280¼, Macherey-Nagel, Düren, Germany). The filtrate was analyzed colorimetrically at 645 nm wavelength (Specord 205, Analytik Jena, Germany) using malachite green oxalate (Sigma, St. Louis, MO, United States) as reagent according to the procedure described by Lanzetta et al. (1979) (link).
Total P (P_tot) was extracted from about 50 mg of dried, milled soil and plant material in 2 ml 65% HNO₃ at 160°C for 12 h according to Heinrichs et al. (1986) . Extracted samples were filtered using phosphate free filter paper (MN 280¼, Macherey-Nagel, Düren, Germany), and used for elemental analysis by inductively coupled plasma–optical emission spectroscopy (ICP–OES) (iCAP 6000 Series ICP–OES, Thermo Fisher Scientific, Dreieich, Germany). Bark and xylem exudates were directly used after filtration for P determinations.

Zavišić A., Yang N., Marhan S., Kandeler E, & Polle A. (2018). Forest Soil Phosphorus Resources and Fertilization Affect Ectomycorrhizal Community Composition, Beech P Uptake Efficiency, and Photosynthesis. Frontiers in Plant Science, 9, 463.

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Radiolabeled Oleic Acid Uptake Protocol

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The [1-14 C]Oleic acid (1.85 GBq mmol -1 ) was purchased from PerkinElmer (Waltham, MA, USA). Fetal bovine serum was purchased from Biowest (Nuaille, France). Dulbecco's modified Eagle's medium and Hank's buffered salt solution were purchased from Pharmaceutical (Tokyo, Japan). GIT medium was from Nippon Seiyaku (Tokyo, Japan). Penicillin (10 000 units ml -1 ), streptomycin (10 000 mg ml -1 ) and glutamine (200 mm) solution were from Invitrogen (Carlsbad, CA, USA). Phosphatidylcholine, phosphatidylserine, dicetylphosphate, cholesterol, Ham's F-12 medium, malachite green oxalate and BFA 1 were purchased from Sigma-Aldrich (St Louis, MO, USA). Perchloric acid and Triton X-100 were purchased from Wako (Osaka, Japan).

Uchida R., Nakajyo K., Kobayashi K., Ohshiro T., Terahara T., Imada C, & Tomoda H. (2016). 7-Chlorofolipastatin, an inhibitor of sterol O-acyltransferase, produced by marine-derived Aspergillus ungui NKH-007. The Journal of antibiotics, 69(8).

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Malachite Green Aptamer Binding Assay

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RNA aptamer (MGA) (5'-GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC-3') was purchased from AITbiotech Pte Ltd Singapore. Malachite green oxalate (MG) and malachite green carbinol base (MGOH) were purchased from Sigma Aldrich, Singapore.
Dimethyl sulfoxide (DMSO) was purchased from Alfa Aesar, USA. Ethanol (EtOH)
was purchased from Fisher Scientific, Singapore. HPLC grade acetonitrile (ACN) was purchased from Tedia, USA. Polystyrene microplates (96-well) were purchased from Corning, USA. All chemicals and materials were used as received.

Zhou Y., Chi H., Wu Y., Marks R.S, & Steele T.W.J. (2016). Organic additives stabilize RNA aptamer binding of malachite green. Talanta, 160.

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