siRNAs specific to Numb (SASI_Hs01_00245423, 5′GAUAGUGUUGGUGCAUCA, 3′UGAUGAACCAACGACUAUC), SRPK1, SRPK2, c-Myc, Oct4, Rbfox1, Rbfox2, Rbfox3, Hsp90 and negative control siRNA were purchased from Sigma-Aldrich. Transfection was performed using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions. A final concentration of 20 nM siRNA duplex was used for each transfection.
Srpk2
Manufactured by Merck Group
Sourced in United Kingdom
SRPK2 is a laboratory equipment product manufactured by Merck Group. It is a serine/arginine-rich protein kinase 2 that plays a role in the regulation of gene expression. The core function of SRPK2 is to phosphorylate serine and arginine-rich splicing factors, which are involved in the processing of messenger RNA.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
C myc, by Merck Group (1 mentions)
Hsp90, by Merck Group (1 mentions)
Rbfox2, by Merck Group (1 mentions)
Hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Sc 35, by Abcam (1 mentions)
2 protocols using srpk2
1
Targeted siRNA Knockdown of Key Regulators
2
Quantitative Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blotting was performed on total proteins from patient tissues, cultured cells and xenograft tumors tissues. The protein was separated by dodecyl sulfate, sodium salt-polyacrylamide gel electrophoresis (SDS-PAGE), then the protein expressions were detected using standard techniques for immunoassays. Antibodies to CyclinE (SAB, #29030, 1:1000 dilution), SRPK2 (Sigma-Aldrich, #HPA015522, 1:1000 dilution), p45SKP2 (Invitrogen, #323300, 1:2000 dilution), SC35 (Abcam, Cambridge, UK, #ab204916, 1:2000 dilution), E2F1 (Abcam, #ab179445, 1:2000 dilution). HRP-conjugated secondary antibodies (Invitrogen, G-21040) were used for the blotting. Controls were referred to β-actin (Cell Signaling Technology, Danvers, MA, USA; #4970, 1:5000 dilution). The expression of each protein was analyzed using biometric digital image software and recorded as integral density (ID). The final result of the ratio of target proteins were calculated as ID (per protein) / ID (β-actin) protein expression.
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