Lc ac system

The sample was analyzed with liquid chromatography−electrospray ionization−tandem mass spectrometry (LC–ESI–MS/MS) using an Exion LC AC system for separation and a SCIEX Triple Quad 5500 + MS/MS system with ESI for detection. For the separation, a ZORBAX Eclipse Plus C18 Column (4.6100 mm, 1.8 m) was used. The mobile phase was 0.1% formic acid in water (solvent A) and acetonitrile (solvent B) with the following gradient: 2% B from 0–1 min, 2–60% B from 1–8 min, 60% B from 8–12 min, and 2% B from 12.01–15 min. The flow rate was 0.8 mL/min and the injection volume was 3 µL. The selected polyphenols were analyzed using the negative ionization mode using Multiple Reaction Monitoring (MRM). The curtain gas was 25 psi, the ion spray voltage was 4500, the source temperature was 400 °C, the ion source gases 1 and 2 were 55 psi with a declustering potential of 50, the collision energy was 25 eV and the collision energy spread was 10 eV.

The analysis of crude propolis, nano-capsules propolis extracts by slovent and SCF-CO₂ at 50 °C was performed using liquid chromatography–electro-spray ionization–tandem mass spectrometry (LC–ESI–MS/MS) with an Exion LC/AC system for separation and SCIEX Triple Quad 5500+ MS/MS system equipped with an electro-spray ionization (ESI) for detection. The instrument data were collected and processed using the SCIEX OS 1.6.10.40973 software.
The separation of the targeted analyses was performed with a Discovery^® BIO Wide Pore C18-5 Column (4.6 × 250 mm, 5 µm). The mobile phases were consisted of two eluents A: 0.1% formic acid in water; B: 0.1% formic acid in acetonitrile (LC grade). The mobile phase gradient was programmed as follows: 5% B at 0 min, 5–25% B from 0.0 to 60.0 min, 25–5% B from 60 to 65 min, 5% from 65 to 70. The flow rate was 1.0 ml/min and the injection volume was 5 µl. For MS/MS analysis, negative ionization mode was applied with a scan (EMS-IDA-EPI) from 150 to 800 Da with the following parameters: curtain gas: 25 psi; ion spray voltage: −4500; source temperature: 400 °C; ion source gas 1 & 2 were 55 psi.