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Agencourt rnaadvance

Manufactured by Beckman Coulter
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The Agencourt RNAadvance is a nucleic acid extraction solution designed to efficiently isolate high-quality RNA from a variety of sample types. It utilizes magnetic bead-based technology to capture and purify RNA, enabling consistent and reliable extraction.

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Lab products found in correlation

Quant it ribogreen rna assay, by Thermo Fisher Scientific (2 mentions) Nextseq 500 instrument, by Illumina (2 mentions) Ribo zero rrna removal beads, by Illumina (2 mentions) T prep method, by Covaris (2 mentions) Truseq stranded total rna kit, by Illumina (2 mentions) Allprep dna rna ffpe kit, by Qiagen (2 mentions) Agilent rna 6000 nano kit, by Agilent Technologies (2 mentions) Biomek fxp automation, by Beckman Coulter (2 mentions)

2 protocols using agencourt rnaadvance

1

RNA Extraction from Frozen and FFPE Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
A small piece of each snap-frozen primary tumor was pulverized using the T-prep method (Covaris, cat # 520097) on dry ice, followed by implementation of Agencourt RNAadvance (Beckman Coulter, cat # A32645) on a Biomek® FXP automation workstation (Beckman Coulter) to isolate RNA. We used 150 ng RNA to prepare libraries with TruSeq Stranded Total RNA kits (Illumina, cat #RS-122–2301). From FFPE tissues, we deparaffinized four 10-μm sections, rehydrated these in an ethanol/water series, and extracted RNA using AllPrep DNA/RNA FFPE kits (Qiagen). We measured concentrations using the Quant-iT RiboGreen RNA assay (Thermo Fisher) and assessed quality on an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit. Ribosomal and mitochondrial RNAs were removed using biotinylated, target-specific oligonucleotides combined with Ribo-Zero rRNA removal beads and the TruSeq Stranded Total RNA kit (Illumina). 75-bp single-end reads were sequenced on a NextSeq 500 Instrument (Illumina).
Cejas P., Drier Y., Dreijerink K.M., Brosens L.A., Deshpande V., Epstein C.B., Conemans E.B., Morsink F.H., Graham M.K., Valk G.D., Vriens M.R., Fernandez-del Castillo C., Ferrone C., Adar T., Bowden M., Whitton H.J., Da Silva A., Font-Tello A., Long H.W., Gaskell E., Shoresh N., Heaphy C.M., Sicinska E., Kulke M.H., Chung D.C., Bernstein B.E, & Shivdasani R.A. (2019). Enhancer signatures stratify and predict outcomes of non-functional pancreatic neuroendocrine tumors. Nature medicine, 25(8), 1260-1265.
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2

RNA Extraction from Frozen and FFPE Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
A small piece of each snap-frozen primary tumor was pulverized using the T-prep method (Covaris, cat # 520097) on dry ice, followed by implementation of Agencourt RNAadvance (Beckman Coulter, cat # A32645) on a Biomek® FXP automation workstation (Beckman Coulter) to isolate RNA. We used 150 ng RNA to prepare libraries with TruSeq Stranded Total RNA kits (Illumina, cat #RS-122–2301). From FFPE tissues, we deparaffinized four 10-μm sections, rehydrated these in an ethanol/water series, and extracted RNA using AllPrep DNA/RNA FFPE kits (Qiagen). We measured concentrations using the Quant-iT RiboGreen RNA assay (Thermo Fisher) and assessed quality on an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit. Ribosomal and mitochondrial RNAs were removed using biotinylated, target-specific oligonucleotides combined with Ribo-Zero rRNA removal beads and the TruSeq Stranded Total RNA kit (Illumina). 75-bp single-end reads were sequenced on a NextSeq 500 Instrument (Illumina).
Cejas P., Drier Y., Dreijerink K.M., Brosens L.A., Deshpande V., Epstein C.B., Conemans E.B., Morsink F.H., Graham M.K., Valk G.D., Vriens M.R., Fernandez-del Castillo C., Ferrone C., Adar T., Bowden M., Whitton H.J., Da Silva A., Font-Tello A., Long H.W., Gaskell E., Shoresh N., Heaphy C.M., Sicinska E., Kulke M.H., Chung D.C., Bernstein B.E, & Shivdasani R.A. (2019). Enhancer signatures stratify and predict outcomes of non-functional pancreatic neuroendocrine tumors. Nature medicine, 25(8), 1260-1265.
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