Glycerophosphocholine

Manufactured by Merck Group

Sourced in Germany

Glycerophosphocholine is a chemical compound that serves as a common component in various laboratory applications. It is a water-soluble molecule consisting of a glycerol backbone, a phosphate group, and a choline moiety. Glycerophosphocholine is widely used in biochemical research and analysis, often as a standard or reference material.

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Lab products found in correlation

Betaine hydrochloride, by Merck Group (2 mentions) N n dimethylglycine, by Merck Group (2 mentions) Acetonitrile, by Merck Group (1 mentions) Cofilin, by Cell Signaling Technology (1 mentions) L phenylalanine, by Merck Group (1 mentions) P cofilin, by Cell Signaling Technology (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Mmp 2, by Cell Signaling Technology (1 mentions) Mmp 9, by Cell Signaling Technology (1 mentions) Mcf 10a, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Acetonitrile acn, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions)

3 protocols using glycerophosphocholine

Choline-Based Compound Quantification Protocol

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Chloroform (HPLC grade) was from Baker (Deventer, The Netherlands). Methanol, acetonitrile, trifluoroethanol, and water (analytical grade) were from Fluka Analytical/Sigma–Aldrich (Munich, Germany). Choline chloride (>99%), phosphocholine, glycerophosphocholine, betaine hydrochloride, N,N-dimethylglycine were from Sigma–Aldrich (Munich, Germany). D4-choline (Choline-1,1,2,2-d4) chloride as an internal standard was purchased from CDN Isotopes Inc. (Pointe-Claire, QC, Canada). Internal phospholipid standard 1,2-diarachidoyl-sn-glycero-3-phosphocholine (dipalmitoyl-phosphatidylcholine, PC20:0/20:0) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). The purity of the chemicals was checked by liquid chromatography heated electrospray ionization tandem mass spectrometry (LC-H-ESI-MS/MS) (see below). All further chemicals were of analytical grade and from various commercial sources.

Shunova A., Böckmann K.A., Minarski M., Franz A.R., Wiechers C., Poets C.F, & Bernhard W. (2020). Choline Content of Term and Preterm Infant Formulae Compared to Expressed Breast Milk—How Do We Justify the Discrepancies?. Nutrients, 12(12), 3815.

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Choline and Phospholipid Profiling

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Chloroform (HPLC grade) was from Baker (Deventer, the Netherlands). Methanol, acetonitrile, water (analytical grade), choline chloride (>99%), phosphocholine, glycerophosphocholine, betaine hydrochloride and N,N-dimethylglycine were from Sigma-Aldrich (Munich, Germany). D₄-choline (Choline-1,1,2,2-d₄) chloride and D9-choline (N,N,N-trimethyl-d₉) chloride were purchased from CDN Isotopes Inc (Pointe-Claire, Quebec, Canada). 1,2-diarachidoyl-sn-glycero-3-phosphocholine (PC20:0/20:0) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (PE14:0/14:0) were from Avanti Polar Lipids (Alabaster, AL, USA). All further chemicals were of analytical grade and from various commercial sources.

Bernhard W., Raith M., Shunova A., Lorenz S., Böckmann K., Minarski M., Poets C.F, & Franz A.R. (2022). Choline Kinetics in Neonatal Liver, Brain and Lung—Lessons from a Rodent Model for Neonatal Care. Nutrients, 14(3), 720.

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Profiling Cancer Cell Metabolism and Signaling

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MCF‐10A, MDA‐MB‐231, MDA‐MB‐468 and MCF‐7 cells were obtained from ATCC (Shanghai, China). The cells were grown in DMEM (Invitrogen, Carlsbad, CA, USA) under 5% CO₂. MDA‐MB‐231 and MCF‐7 cells were grown in charcoal‐stripped serum containing media for 24 hours, before treatment with indicated concentrations of 9‐cis‐retinoic acid (9‐cisRA; KGaA Merck, Darmstadt, Germany). Antibodies to cofilin, P‐cofilin, MMP2, MMP9, aldehyde dehydrogenase 1 family member A1 (ALDH1A1) and β‐actin were from Cell Signaling Technology, Inc. (Beverly, MA, USA).
Formic acid and acetonitrile (ACN) were obtained from Merck (KGaA Merck). All standard (l‐phenylalanine, glycerophosphocholine, chenodeoxycholic acid glycine conjugate and lysophosphatidylcholine [lysoPC] [18:0]) preparations were purchased from Sigma‐Aldrich (St Louis, MO, USA).
The ultra‐performance liquid chromatography/mass spectroscopy (UPLC/MS) portion of the platform is based on an Accela UPLC (Thermo Fisher Scientific Corporation, Waltham, MA, USA) and a LTQ‐Orbitrap XL hybrid mass spectrometer (Linear Ion Trap mass spectrometer with Orbitrap; Thermo Fisher Scientific Corporation). The GC/MS portion is based on an Agilent 7890A Series GC and a QQQ mass spectrometer (Triple Quadrupole mass spectrometer; Thermo Fisher Scientific Corporation).

Wu J., Yang R., Zhang L., Li Y., Liu B., Kang H., Fan Z., Tian Y., Liu S, & Li T. (2018). Metabolomics research on potential role for 9‐cis‐retinoic acid in breast cancer progression. Cancer Science, 109(7), 2315-2326.

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