The GS was weighed and homogenized in PBS of 4°C, followed by centrifugation at 12000 g for 5 min to get the supernatant for further test. Malondialdehyde (MDA) content, superoxide dismutase activity (Mn-SOD, Cu-Zn SOD, and t-SOD), and catalase (CAT) activity were determined with commercial assay kits produced by Nanjing Jiancheng Bioengineering Institute, China. Total antioxidant capacity was measured by ABTS assay kits purchased from Beyotime Biotechnology, China. The total protein content of each sample was measured by the Bradford method to normalize the results.
Abts assay kit
Manufactured by Beyotime
Sourced in China
The ABTS assay kit is a laboratory tool used for the quantitative analysis of antioxidant capacity. The kit utilizes the ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical cation decolorization assay, which is a widely recognized method for evaluating the ability of compounds to scavenge free radicals and other reactive species.
Automatically generated - may contain errors
Lab products found in correlation
Folin ciocalteu reagent, by Sinopharm (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Sinopharm (1 mentions)
Fluorescein sodium salt, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Thermo Fisher Scientific (1 mentions)
Acetonitrile and methanol, by Thermo Fisher Scientific (1 mentions)
Alcalase 2.4l, by Novo Nordisk (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
Flexstation 3 plate reader, by Molecular Devices (1 mentions)
6 protocols using abts assay kit
1
Antioxidant Activity Assay Protocol
2
Antioxidant Activity of Aged Vinegar
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The FRAP and ABTS assay kits were supplied from Beyotime Institute of Biotechnology (Shanghai, China), which were used to measure antioxidant activity. Standards of organic acids and phenolic compounds were purchased from Sigma-Aldrich (Deisenhofen, Germany). Folin-Ciocalteu reagent and DPPH were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
6 vinegar samples for each aging time (0, 3, 6, 8 and 10 years) were obtained from Zhenjiang Hengshun Vinegar Co., Ltd (Jiangsu, China). The characteristics of the vinegar samples were shown inTable 6 .
6 vinegar samples for each aging time (0, 3, 6, 8 and 10 years) were obtained from Zhenjiang Hengshun Vinegar Co., Ltd (Jiangsu, China). The characteristics of the vinegar samples were shown in
3
Wheat Gluten Enzymatic Hydrolysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Wheat gluten was obtained from CF Haishi Biotechnology Ltd. Co. (Beijing, China) and kept in sealed plastic bags at room temperature until use. Alcalase 2.4L (2.4 AU/g) was purchased from Novo Nordisk (Copenhagen, Denmark). Protex 7L (1600 AU/g) and α-amylase (26,000 RAU/g) were bought from Genencor Division of Danisco (Rochester, NY, USA). Fluorescein sodium salt, Trolox, DPPH, 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH), hippuryl-histidyl-leucine (HHL), hippuric acid (HA), and ACE (from rabbit lung) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The ABTS assay kit was supplied by Beyotime Biotechnology (Haimen, China). Acetonitrile and methanol were provided by Fisher Scientific (Pittsburgh, PA, USA). Trifluoroacetic acid (TFA) was supplied by Alfa Aesar (Ward Hill, MA, USA). Synthesized peptides were obtained from Scilight Biotechnology Co., Ltd (Beijing, China). The purity of the synthesized peptides was more than 98% according to HPLC analysis. All other chemicals were of analytical grade.
4
Antioxidant Activity Measurement by ABTS Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antioxidant activities of P1 and P2 were measured by ABTS assay kit (Beyotime) according to the manufacturer’s protocols34 (link). The absorbance at 734 nm was measured with a FlexStation 3 Plate Reader (Molecular Devices, USA). The data were expressed as TE/g of samples. The equation is shown as below:
5
Colorimetric Assay for Total Antioxidant Capacity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total antioxidant capacity (TAC) was determined by using the colorimetric ABTS assay kit (Beyotime Institute of Biotechnology, Haimen, China) according to the method described by Miller et al. (1993 (link)). In this method, the sample antioxidants inhibit peroxidase-mediated oxidation of 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonate, colorless solution) to the radical cation (blue-green). TAC was expressed as mmol Trolox equiv/L.
6
ABTS Radical-Scavenging Activity of EMOS-1a
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ABTS radical-scavenging activity of EMOS-1a was determined with an ABTS assay kit (Beyotime Biotechnology Co., Ltd., Shanghai, China). ABTS working liquor was prepared by reacting ABTS stock solution with potassium persulfate solution at a 1:1 volume ratio. The mixture was stored at room temperature for 12–16 h in the dark before use and left to stabilize for 2 to 3 days. The concentration of the resulting blue-green ABTS radical solution was adjusted to give an absorbance of 0.7 ± 0.05 at 734 nm. A total of 200 µL of ABTS working solution and 10 µL EMOS-1a solution were mixed in 96-well plates, which were protected from light. Absorbance was measured at 734 nm after 2–6 min of incubation at room temperature. Antioxidant activity was expressed as the Trolox-equivalent antioxidant capacity of the EMOS-1a solution.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!