The morphologies of HMSNs and HMSNs–S–S–CPA–CytC–LA were characterized by transmission electron microscopy (TEM, LIBRA 200 CS; Carl Zeiss Co., Germany) and scanning electron microscopy (FEI-SEM, Nova 400; USA). Surface areas and pore sizes distribution of various HMSNs were characterized by Brunauer–Emmett–Teller (BET) and Barrett–Joyner–Halenda (BJH) (ASAP2020M; USA) analyses, respectively. The potential and dimension distribution of different samples were characterized by Zeta potential measurements (Nano ZS90 Zetasizer; Malvern Instruments Co. Ltd, UK) with dynamic light scattering (DLS) at 25°C. Fourier transform infrared spectroscopy (FTIR, model 6300; Bio-Rad Co. Ltd, USA) and thermogravimetric analysis (TGA, DTG 60H; Japan) were employed to monitor the functionalization procedures.
Libra 200 cs
Manufactured by Zeiss
Sourced in Germany
The LIBRA 200 CS is a transmission electron microscope (TEM) designed and manufactured by Zeiss. It provides high-resolution imaging and analytical capabilities for materials science research and applications. The LIBRA 200 CS features advanced electron optics and a cold field emission gun, enabling it to produce high-quality images and perform detailed elemental analysis of samples.
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3 protocols using libra 200 cs
1
Characterization of Hierarchical Mesoporous Silica Nanoparticles
2
Characterization of Cu-based Hydrogels
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The morphology of the Cu COF and hydrogels was observed via TEM and SEM (LIBRA 200 CS, Carl Zeiss Co., Germany).
The compressive mechanical characteristics of the blank gel and 4-OI@Cu@Gel (3 mm thick) were detected using a MACH-1 Micromechanical System (Biomomentum, Inc., Canada). The compressive strength was calculated by using the following equation: F = P/A, where F refers to the compressive strength, P refers to the maximum load, A refers to the cross-section of the area.
For the degradation test, the blank gel and 4-OI@Cu@Gel were weighed and incubated in PBS at 37 °C. At predetermined time intervals, the hydrogels were removed from PBS, dried at 37 °C, and weighed.
For the drug release test, the 4-OI@Cu@Gel was incubated in PBS at 37 °C. The supernatant was collected at different time points. The amount of 4-OI was determined by multimode plate reader. The amount of Cu2+ was measured by a Cu2+ detection kit (Leagene Biotechnology, Beijing, China) following the manufacturer's instructions.
For rheological test, time sweep was performed on a rheometer (MCR301, Anton Paar Co., Austria).
The compressive mechanical characteristics of the blank gel and 4-OI@Cu@Gel (3 mm thick) were detected using a MACH-1 Micromechanical System (Biomomentum, Inc., Canada). The compressive strength was calculated by using the following equation: F = P/A, where F refers to the compressive strength, P refers to the maximum load, A refers to the cross-section of the area.
For the degradation test, the blank gel and 4-OI@Cu@Gel were weighed and incubated in PBS at 37 °C. At predetermined time intervals, the hydrogels were removed from PBS, dried at 37 °C, and weighed.
For the drug release test, the 4-OI@Cu@Gel was incubated in PBS at 37 °C. The supernatant was collected at different time points. The amount of 4-OI was determined by multimode plate reader. The amount of Cu2+ was measured by a Cu2+ detection kit (Leagene Biotechnology, Beijing, China) following the manufacturer's instructions.
For rheological test, time sweep was performed on a rheometer (MCR301, Anton Paar Co., Austria).
3
Comprehensive Characterization of ZPM@PDE
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The PDEA-bPEI was characterized by 1H NMR (400 MHz Agilent) and Fourier transform infrared (FT-IR) spectrophotometer (PerkinElmer S100, United States). Transmission electron microscopy (TEM, LIBRA 200 CS, Carl Zeiss Co., Germany), X-ray powder diffraction (XRD, PANalytical, Netherlands), thermal gravimetric analysis (TGA, Mettler Toledo, Switzerland), and N2 adsorption-desorption (ASAP 2460, Micromeritics, United States) were used to characterize the ZPM@PDE. The particle size and ζ-potential of ZPM@PDE were investigated by dynamic light scattering (DLS) (Nano ZS90 Zeta sizer, Malvern Instruments Co., Ltd., United Kingdom).
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