CCP (500 μg/mL) was analyzed in the wavelength range of 200–600 nm by an Evolution 300 UV–VIS spectrometer (Thermo Scientific) to detect the presence of nucleic acids and proteins. The organic function groups of CCP were identified in the spectrophotometer range of 4000–400 cm−1 by FT-IR. CCP (1.5 ± 0.5% mg) mixed with 100 mg of spectroscopic grade KBr powder was milled accurately, then pressed into a 1 mm pellet for FT-IR analysis via VERTEX 70 FT-IR infrared spectrometer (Bruker, Germany).
Evolution 300 uv vis spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Evolution 300 UV-Vis spectrometer is a laboratory instrument designed for measuring the absorption of ultraviolet and visible light by samples. It is capable of analyzing a wide range of sample types, including liquids, solids, and gases. The instrument provides accurate and reliable data, making it a useful tool for applications such as chemical analysis, material science, and life science research.
Automatically generated - may contain errors
Lab products found in correlation
Vertex 70 ft ir infrared spectrometer, by Bruker (1 mentions)
Jsm 6510, by JEOL (1 mentions)
Cary eclipse fluorescence spectrometer, by Agilent Technologies (1 mentions)
Avance 600, by Bruker (1 mentions)
Zorbax 300sb c18 column, by Agilent Technologies (1 mentions)
Silica gel, by Qingdao Haiyang Chemical (1 mentions)
Acquity uplc system, by Waters Corporation (1 mentions)
Agilent 1100 hplc, by Agilent Technologies (1 mentions)
Uplc q tof ms, by Agilent Technologies (1 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
Trypsin edta solution, by Merck Group (1 mentions)
7 protocols using evolution 300 uv vis spectrometer
1
Spectroscopic Characterization of Chitosan Carboxypeptidase
2
UV Absorption Analysis of UPPs
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The UV absorption (the wavelength range of 190–400 nm) of UPPs 1–3 (1 mg of each) was analyzed based on an Evolution 300 UV–VIS spectrometer (ThermoFisher Scientific, MA, U.S.A.) compared with the blank control (distilled water).
3
Biophysical Characterization of Photosynthetic Complexes
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An Evolution 300 UV/vis spectrometer (Thermo Scientific, Germany) was used in the range from 350 to 750 nm at a rate of 600 nm min−1 for the absorbance measurements. Scanning electron microscopy (SEM) was executed with a JSM-6510 (JEOL, Japan). Fluorescence was studied after excitation at the absorbance maxima of the dyes, 532 and 590 nm, respectively. A Cary Eclipse Fluorescence Spectrometer (Varian, USA) was utilized.
The O2 consumption of the constructs was measured in solution in buffer G (5 μM chlorophyll in 5 mM tris pH 7, 16 μM cyt c, 0.2 mg mL−1 sodium ascorbate, 0.05 mg mL−1 methyl viologen, and 0.02% DDM) with a Clark-type electrode (Oxygraph+, Hansatech, Germany) as reported previously by application of cyt c as an electron donor to the luminal side of PSI.60 (link)
The O2 consumption of the constructs was measured in solution in buffer G (5 μM chlorophyll in 5 mM tris pH 7, 16 μM cyt c, 0.2 mg mL−1 sodium ascorbate, 0.05 mg mL−1 methyl viologen, and 0.02% DDM) with a Clark-type electrode (Oxygraph+, Hansatech, Germany) as reported previously by application of cyt c as an electron donor to the luminal side of PSI.60 (link)
4
Analytical Instrumentation for Natural Product Characterization
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NMR spectra were recorded
by a Bruker AVANCE-600 (600 MHz) instrument (Bruker Biospin, Zurich,
Switzerland). UV spectra were recorded by an Evolution 300 UV–vis
spectrometer (Thermo Fisher Scientific). UPLC-TQD-MS was operated
using an Acquity UPLC system (Waters Corporation, Milford, MA, USA)
coupled with a MS (Xevo TQD, Waters MS Technologies, Manchester, UK).
HRESIMS spectra were obtained from UPLC-QTOF-MS (Agilent Inc., Santa
Clara, CA, USA). HPLC separations used the Agilent 1100 HPLC equipped
with a UV detector with a semipreparative column (Zorbax 300 SB-C18
column, 9.4 mm × 25 cm, 4 μm). Sephadex LH-20 (25–100
μm) was purchased from Pharmacia Fine Chemicals (Piscataway,
NJ), and HPLC-MS grade acetonitrile, water, and formic acid were purchased
from J. T. Baker (Phillipsburg, NJ, USA). Thin-layer chromatography
(TLC) silica gel plates and a silica gel of 200–300 mesh were
obtained from Qingdao Haiyang Chemical Co. Ltd. (P. R. China), and
all reagents were analytical grade (Guangzhou Chemical Reagent Factory,
P. R. China).
by a Bruker AVANCE-600 (600 MHz) instrument (Bruker Biospin, Zurich,
Switzerland). UV spectra were recorded by an Evolution 300 UV–vis
spectrometer (Thermo Fisher Scientific). UPLC-TQD-MS was operated
using an Acquity UPLC system (Waters Corporation, Milford, MA, USA)
coupled with a MS (Xevo TQD, Waters MS Technologies, Manchester, UK).
HRESIMS spectra were obtained from UPLC-QTOF-MS (Agilent Inc., Santa
Clara, CA, USA). HPLC separations used the Agilent 1100 HPLC equipped
with a UV detector with a semipreparative column (Zorbax 300 SB-C18
column, 9.4 mm × 25 cm, 4 μm). Sephadex LH-20 (25–100
μm) was purchased from Pharmacia Fine Chemicals (Piscataway,
NJ), and HPLC-MS grade acetonitrile, water, and formic acid were purchased
from J. T. Baker (Phillipsburg, NJ, USA). Thin-layer chromatography
(TLC) silica gel plates and a silica gel of 200–300 mesh were
obtained from Qingdao Haiyang Chemical Co. Ltd. (P. R. China), and
all reagents were analytical grade (Guangzhou Chemical Reagent Factory,
P. R. China).
5
Dissolution Rates of ST-A and ST-B
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The dissolution rates of ST-A and ST-B in distilled water and gastric juice pH 1.2 acid solution (in the presence of 0.2% SLS) at 37 °C were measured using a Thermo Scientific Evolution 300 UV-Vis spectrometer (Thermo Scientific, Waltham, MA, USA). For dissolution rate measurement, an excess quantity of drug was poured into 200 mL of water or gastric juice pH 1.2 acid solution, which was pre-heated to 37 °C and rotated at 150 rpm. The mixture was stirred for 120 min, and at specific time intervals, 2 mL of the sample was withdrawn and replaced with 2 mL of a fresh medium to maintain a total volume. The sample was then filtered through Whatman’s 0.45 μm syringe filter. The absorbance at λmax of the obtained solution was determined. The concentrations of ST-A and ST-B were achieved by calculations based on the standard curves of Sor-Tos. Each concentration was measured three times in parallel and the average value was obtained.
6
Dextranase Kinetics of Dex-TO Nanoparticles
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Example 2
Dex-TO solution was prepared at 2.2 μM (0.26 μmol isomaltose unit in 1 ml of 1×DPBS pH 7.4). 5 units of 400-800 units/mg lyophilized dextranase from Penicillium sp. (Sigma-Aldrich, St. Louis, Mo.), was prepared in 6 μl 1×DPBS, and added to the Dex-TO solution. Reaction kinetics were measured by UV spectra from 250 nm to 750 nm, on an Evolution 300 UV-Vis spectrometer (Thermo Scientific), and recorded at 0, 1, 2, and 20 hours after incubation at 37° C. The TO concentration was calculated according to the absorbance at 509 nm and an extinction coefficient of 63,000 M−1 cm−1 as described in Nygren J, et al. Biopolymers. 1998; 46: 39-51. An average of 2.8 TOs were present per nanoparticle
7
Measuring ADN and FH-Cy5.5 Nanoparticles
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