L.E. LUVs treated with 1TAT, L.E. LUVs treated with 3TAT, and PG LUVs treated with 3TAT were frozen in vitreous ice on a Quantfoil R2/1 holey carbon grid with an FEI Vitrobot respectively. Cryo-EM images were acquired on a K2 Summit Direct-detection camera (Gatan) in the electron-counting mode using a TECNAI F20 cryo-electron microscope (FEI) operated at 200 kV. A nominal magnification of 19000× or 7800× was used, giving a pixel size of 1.87 Å/pixel or 4.8 Å/pixel, respectively.
Tecnai f20 cryo electron microscope
Manufactured by Thermo Fisher Scientific
The Tecnai F20 cryo-electron microscope is a high-resolution transmission electron microscope designed for cryogenic imaging of biological samples. It is capable of capturing high-quality images of macromolecular structures and cellular components at the nanoscale level. The Tecnai F20 is equipped with advanced features and optics, enabling researchers to study the structure and function of a wide range of biological samples in their native state.
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Lab products found in correlation
4 protocols using tecnai f20 cryo electron microscope
1
Cryo-EM analysis of lipid vesicles
2
Cryo-EM Imaging of UC-MSC Extracellular Vesicles
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UC‐MSC EV samples were deposited on an electron microscopy (EM) grid coated with a perforated carbon film. Samples were quickly frozen in liquid nitrogen‐cooled liquid ethane using a Leica EM‐PC cryo system. EM grids were maintained under liquid nitrogen until use. EM grids transferred to a Tecnai F20 cryo‐electron microscope (FEI, ThermoFisher) operating at 200 kV. Grids were mounted in a Gatan 626 cryo‐holder and images were recorded with a FEI‐Eagle camera by Prof. Alain Brisson (University of Bordeaux, France)
3
Cryo-EM Imaging of MSC-Derived Extracellular Vesicles
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For each MSC‐EV sample, 4 μL was deposited on an electron microscopy (EM) grid coated with a perforated carbon film. Samples were quickly frozen by plunging in liquid ethane cooled by liquid nitrogen, using a Leica EM‐PC cryo system. EM grids were stored in cryo‐boxes maintained under liquid nitrogen, until the observation in the electron microscope. EM grids were mounted in a Gatan 626 cryo‐holder, transferred in a Tecnai F20 cryo‐electron microscope (FEI, ThermoFisher) operating at 200 kV. Images were recorded with a FEI‐Eagle camera.
4
Cryo-EM Analysis of UC-MSC-EVs
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UC-MSC-EV samples were deposited on an electron microscopy (EM) grid coated with a perforated carbon film. Samples were quickly frozen in liquid nitrogen-cooled liquid ethane using a Leica EM-PC cryo system. EM grids were maintained under liquid nitrogen until use. EM grids transferred to a Tecnai F20 cryo-electron microscope (FEI, ThermoFisher) operating at 200 kV. Grids were mounted in a Gatan 626 cryo-holder and images were recorded with a FEI-Eagle camera.
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