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Fast airyscan detector

Manufactured by Zeiss
Sourced in Germany

The Fast Airyscan detector is a high-speed and high-resolution imaging tool developed by Zeiss. It is designed to capture images with improved signal-to-noise ratio and enhanced spatial resolution. The core function of the Fast Airyscan detector is to enable rapid data acquisition and efficient image processing.

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2 protocols using fast airyscan detector

1

Confocal Microscopy of Fluorescent Cells

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Cells transfected with fluorescent reporter plasmid were prepared for microscopy by transferring 200 µl of cells to a glass-bottom dish or glass-bottom 8-well chamber (Ibidi). Confocal microscopy was performed on a Zeiss Axio Observer LSM 880 with an Fast Airyscan detector and a 63 x/NA1.40 Plan-Apochromatic oil immersion objective (Carl Zeiss AG, Oberkochen, Germany). Confocal stacks were acquired by frame scanning in superresolution mode, and images were processed using the automated Airyscan algorithm (Zeiss).
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2

Zeiss Airyscan Microscopy Protocol

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Zeiss LSM880 with Fast Airyscan detector or LSM510 (Zeiss, Germany) were used to collect z-stack images. Fast Airyscan was performed at Nyquist sampling and using SR settings. For whole cell analysis, z-stack images were collected to visualize cells from top to bottom, and for live-cell imaging, 3–5 images per z-stack were collected per time point. Airyscan processing (pixel reassignment) was performed in Zen Black software under default settings. Live-cell imaging is described in Supplementary Methods.
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