Ab125015

Manufactured by Abcam

Ab125015 is a lab equipment product offered by Abcam. It is a device designed for laboratory use, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Further information on the intended use of this product is not available.

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Lab products found in correlation

Ab7817, by Abcam (3 mentions) Ab34710, by Abcam (3 mentions) Ab150113, by Abcam (3 mentions) Ab150077, by Abcam (3 mentions) Pa5 28932, by Thermo Fisher Scientific (2 mentions) Pa5 99526, by Thermo Fisher Scientific (2 mentions) Ab216482, by Abcam (1 mentions) Ab28856, by Abcam (1 mentions) Ab2413, by Abcam (1 mentions) D1306, by Thermo Fisher Scientific (1 mentions) Anti il 6, by Cell Signaling Technology (1 mentions) Anti p erk1 2, by Cell Signaling Technology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Uniprotkb, by UniProt (1 mentions) Ab207327, by Abcam (1 mentions) Ab83053, by Abcam (1 mentions) Ab222101, by Abcam (1 mentions) Sc 130920, by Santa Cruz Biotechnology (1 mentions) Af506, by R&D Systems (1 mentions) Ma5 32090, by Thermo Fisher Scientific (1 mentions)

4 protocols using ab125015

IL11 and TGF-β1 Signaling Pathway Analysis

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Recombinant proteins: Mouse TGFβ1 (7666-MB, R&D Systems), mouse IL11 (rmIL11, UniProtKB: P47873, GenScript). Antibodies: anti-smooth muscle actin (ab7817, abcam), anti-Collagen I (ab34710, abcam), anti-IL11RA (ab125015, abcam), goat anti-mouse Alexa Flour 488-conjugated secondary antibody (ab150113, abcam), goat anti-rabbit Alexa Flour 488-conjugated secondary antibody (ab150077, abcam), DAPI (D1306, Thermo Fisher Scientific). Primary antibodies for Western blots include: anti-Fibronectin antibody (ab2413, Abcam); anti-IL6 (12912, Cell Signaling); anti-p-ERK1/2 (4370, Cell Signaling), anti-ERK1/2s (4695, Cell Signaling), anti-p-SMAD2 (ab216482, Abcam), anti-p-NF-kB (ab28856, Abcam), anti-p-STAT3 (4113, Cell Signaling), anti-SMAD2 (5339, Cell Signaling), anti-NF-kB (8242, Cell Signaling), anti-STAT3 (4904, Cell Signaling) and anti-GAPDH (2118, Cell Signaling). Monoclonal anti-IL11 antibody (X203), generated in our previous study^{15 (link)}, was used to detect IL11 protein expression in tissue lysates. Secondary antibodies for Western blots include: anti-rabbit HRP (7074, Cell Signaling) or anti-mouse HRP (7076, Cell Signaling).

Ng B., Widjaja A.A., Viswanathan S., Dong J., Chothani S.P., Lim S., Shekeran S.G., Tan J., McGregor N.E., Walker E.C., Sims N.A., Schafer S, & Cook S.A. (2021). Similarities and differences between IL11 and IL11RA1 knockout mice for lung fibro-inflammation, fertility and craniosynostosis. Scientific Reports, 11, 14088.

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Comprehensive Protein Expression Analysis

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ACTA2 (ab7817, Abcam, 1:1000 for western blot and 1:500 for operetta assay), Albumin (ab207327, Abcam, 1:100 for IF and flow cytometry), Cleaved Caspase-3 (9664, CST, 1:1000), Caspase-3 (9662, CST, 1:1000), Collagen I (ab34710, Abcam, 1:500), phospho-ERK1/2 (4370, CST, 1:1000), ERK1/2 (4695, CST, 1:1000), GAPDH (2118, CST, 1:1000), gp130 (human, PA5-28932, Thermo Fisher, 1:100), gp130 (mouse, PA5-99526, Thermo Fisher, 1:100), gp130 (extracellular, PA5-77476, Thermo Fisher, 1:1000), IgG (11E10, Aldevron), IL6 (AF506, R&D Systems, 1:1000), IL6R (flow cytometry, ab222101, Abcam, 1:100), IL6R (human, IHC and IF, MA1-80456, Thermo Fisher, 1:100), IL6R (mouse, ab83053, Abcam, 1:100), IL11 (X203, Aldevron), IL11RA (inhibition study, X209, Aldevron), IL11RA (IHC, IF, flow cytometry, ab125015, Abcam, 1:100), IL11RA (western blot, sc-130920, Santa Cruz, 1:1000), phospho-JNK (4668, CST, 1:1000), JNK (9252, CST, 1:1000), NOX4 (MA5-32090, Invitrogen, 1:1000), phospho-STAT3 (4113, CST, 1:1000), STAT3 (4904, CST, 1:1000), mouse Alexa Fluor 488 secondary antibody (ab150113, Abcam, 1:200), mouse HRP (7076, CST, 1:2000), rabbit Alexa Fluor 488 secondary antibody (ab150077, Abcam, 1:200), rabbit HRP (7074, CST, 1:2000), rat Alexa Fluor 488 secondary antibody (ab150157, Abcam, 1:200), rat HRP (31470, Santa Cruz, 1:800).

Dong J., Viswanathan S., Adami E., Singh B.K., Chothani S.P., Ng B., Lim W.W., Zhou J., Tripathi M., Ko N.S., Shekeran S.G., Tan J., Lim S.Y., Wang M., Lio P.M., Yen P.M., Schafer S., Cook S.A, & Widjaja A.A. (2021). Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nature Communications, 12, 66.

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Fibrosis Signaling Pathway Analysis

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BIP (3177, CST), Cleaved-Caspase 3 (9664, CST), Caspase 3 (9662, CST), CHOP (5554, CST), Collagen I (ab34710, Abcam), phospho-ERK1/2 (4370, CST), ERK1/2 (4695, CST), GAPDH (2118, CST), neutralizing anti-human gp130 (MAB628, R&D Systems), anti-gp130 (PA5-99526, Thermo Fisher), gp130 (PA5-28932, Thermo Fisher, IF), IgG (11E10, Aldevron), neutralizing anti-IL11 (X203, Aldevron), commercial anti-IL11 (MAB218, R&D Systems), neutralizing anti-IL11RA (X209, Aldevron), IL11RA (ab125015, abcam, IF), phospho-mTOR (2971, CST), mTOR (2972,CST), phospho-p70S6K (9205, CST), p70S6K (2708, CST), p-PDGFRB (3124, CST), PDGFRB (3169, CST), phospho-S6 ribosomal protein (4858, CST), S6 ribosomal protein (2217, CST), ⍺-SMA (ab7817, Abcam, Operetta), ⍺-SMA (19245, CST, WB), phospho-SMAD2 (3108, CST), SMAD2 (5339, CST), phospho-STAT3 (4113, CST), STAT3 (4904, CST), XBP-1 (sc-8015, SantaCruz), mouse Alexa Fluor 488 secondary antibody (ab150113, Abcam), mouse HRP (7076, CST), rabbit Alexa Fluor 488 secondary antibody (ab150077, Abcam), rabbit HRP (7074, CST).

Widjaja A.A., Viswanathan S., Jinrui D., Singh B.K., Tan J., Wei Ting J.G., Lamb D., Shekeran S.G., George B.L., Schafer S., Carling D., Adami E, & Cook S.A. (2021). Molecular Dissection of Pro-Fibrotic IL11 Signaling in Cardiac and Pulmonary Fibroblasts. Frontiers in Molecular Biosciences, 8, 740650.

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Signaling Pathway Protein Analysis

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Phospho ACC Ser79 (11818, CST), ACC (3676, CST), phospho AMPK Thr172 (2535, CST), AMPK (5832, CST) E-Cadherin (3195, CST), phospho-ERK1/2 Thr202/Tyr204 (4370, CST), ERK1/2 (4695, CST), GAPDH (2118, CST), IgG (11E10, Aldevron), neutralizing anti-IL11 (X203, Aldevron), neutralizing anti-IL11RA (X209, Aldevron), IL11RA (ab125015, abcam, IF), phospho LKB1 Ser325 (gifted by Prof. Dario Alessi, University of Dundee), phospho LKB1 Ser428 (3482, CST), LKB1 (3047S), phospho-mTOR Ser2448 (2971, CST), mTOR (2972,CST), phospho-p70S6K Thr389 (9234, CST), p70S6K (2708, CST), phospho P90RSK Ser380 (11989, CST), RSK (9355, CST), phospho-S6 ribosomal protein Ser235/236 (4858, CST), S6 ribosomal protein (2217, CST), SMA (19245, CST), SNAI1 (3879, CST), phospho-STAT3 Tyr705 (4113, CST), STAT3 (4904, CST), phospho ULK1 Ser555 (5869, CST), ULK1 (8054, CST), mouse HRP (7076, CST), rabbit HRP (7074, CST).

Widjaja A.A., Goh J.W.T., Viswanathan S., Tan J., Shekeran S.G., Carling D., Wen L.W., & Cook S.A. (2022). IL11 stimulates ERK/P90RSK to inhibit LKB1/AMPK and activate mTOR in hepatocytes, the stroma and cancer cells.

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