Pe 2400 series 2 chns analyzer

The starting compounds, reagents, and solvents (Sigma-Aldrich, Darmstadt, Germany) used in this study were of analytical reagent grade. Thin-layer chromatography was performed on an aluminum plate precoated with layers of silica gel 60F₂₅₄ (Merck, Darmstadt, Germany). The chromatograms were visualized using UV light. The stationary base in column chromatography (70–230 mesh) was high-purity grade silica gel 60 (Merck, Darmstadt, Germany). Melting points were measured once via a Stuart SMP30 apparatus (Stone, Staffordshire, UK). IR spectra were recorded using KBr pellets on a Satellite FT-IR spectrophotometer (Bruker, Madison, WI, USA). ¹H and ¹³C NMR spectra in DMSO-d₆ were recorded using a Varian Unity Plus (500 MHz) instrument (Varian Medical Systems, Palo Alto, CA, USA). The elemental analyses (%C, H, and N) were determined using a Perkin Elmer PE 2400 Series II CHNS analyzer (Perkin-Elmer, Shelton, CT, USA). The results of the elemental analyses were consistent with the calculated values within the ±0.4% range.

The initial compound (Apollo Scientific, Bredbury, UK) and all reagents and solvents (Sigma-Aldrich, Darmstadt, Germany) were of analytical grade. Thin-layer chromatography was performed on Merck silica gel 60F₂₅₄ plates and visualized using UV light. The stationary phase in column chromatography was high-purity Merck silica gel (pore size 60 Å, 70–230 mesh). Elemental analyses (%C, H, N) of all synthesized compounds in the solid form were carried out using a PerkinElmer PE 2400 Series II CHNS analyzer (Perkin-Elmer, Shelton, CT, USA), the results of which were in agreement with the calculated values within the ±0.4% range. IR spectra were recorded as KBr pellets of the solids using a Satellite FT-IR spectrophotometer (Bruker, Madison, WI, USA). The ¹H and ¹³C NMR spectra in DMSO-d₆ were recorded using Varian Unity Plus (500 MHz) and Varian Gemini (200 MHz) instruments (Varian Medical Systems, Palo Alto, CA, USA). Melting points were determined using a Stuart SMP30 apparatus (Stone, Staffordshire, UK) and were uncorrected.

All of the materials and solvents were of analytical reagent grade. Thin-layer chromatography was performed on Merck (Darmstadt, Germany) silica gel 60F₂₅₄ plates and visualized with UV. The stationary phase for column chromatography was silica gel (60 Å, 70–230 mesh). The results of elemental analyses (%C, H, N) for all of the obtained compounds that were determined on Perkin-Elmer PE 2400 Series II CHNS analyzer (Perkin-Elmer, Shelton, CT, USA) were in agreement with the calculated values within ±0.4% range. ¹H- and ¹³C-NMR spectra in CDCl₃ or DMSO-d₆ were recorded on Varian Unity Plus (500 MHz) and Varian Gemini (200 MHz) instruments (Varian, Palo Alto, CA, USA). ¹H-NMR spectra of selected compounds can be found in Supplementary Materials. IR Spectra (KBr) were determined as KBr pellets of the solids on a Satellite FT-IR spectrophotometer (Mattson Instruments, Madison, WI, USA). Melting points were determined on a Stuart SMP30 apparatus and were uncorrected (Bibby Scientific, Staffordshire, UK).