Cytarabine

CQ (Sigma, St. Louis, MO, USA) was dissolved in Milli Q water. Oligomycin (Sigma), Dex (Wako, Tokyo, Japan), etoposide (Tokyo Kasei, Tokyo, Japan), daunorubicin (Cayman Chemical, MI, USA), cytarabine (LKT Laboratories, St. Paul, MN, USA) and vincristine (LKT Laboratories) were dissolved in dimethyl sulfoxide (DMSO). The final DMSO concentration in every cell culture did not exceed 0.5% (v/v).

Free-base tamoxifen (Sigma-Aldrich T5648) was prepared at 20 mg/ml and administered by oral gavage at 200 mg/kg as previously described.^{58 (link)} Cytarabine (LKT Laboratories C9778; St. Paul, MN, USA) was dissolved in PBS at 20 mg/ml, and doxorubicin (Selleck Chemicals S1208; Houston, TX, USA) was dissolved in PBS and 1% DMSO at 600 μg/ml. Cytarabine and doxorubicin were administered to animals intraperitoneally as previously described.^{5 (link)} Cells treated with 100 nM BafA1 (LCL Labs B-1080; Woburn, MA, USA) were analyzed by immunoblotting as previously described.^{59 (link)} Cells were treated with 100 nM of the Z isomer of 4OHT (LKT Laboratories H9711) to delete Atg5. Cells were treated with ABT-199 (LKT Laboratories A0776), ABT-737 (LKT Laboratories A0778), maritoclax,^{33 (link)} and vorinostat (LKT Laboratories V5734) in 0.5% DMSO for 48 h and subjected to flow cytometric analysis.