Microbial dna qpcr array kit

Multiplex real-time PCR was done using the QIAGEN—Microbial DNA qPCR Array Kit for the detection of a panel of bacterial and fungal pathogens on extracted DNA. The list of the bacteria that were used for the detection included Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus, Escherichia/Shigella spp, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Mycoplasma pneumoniae, Bordetella spp, Chlamydia pneumoniae, Legionella pneumophila, Mycobacterium spp, Mycobacterium avium, Mycobacterium kansasii, Mycobacterium tuberculosis, Nocardia spp, Aspergillus flavus, Aspergillus fumigatus, Pneumocystis jirovecii, PANA—Pan Aspergillus/Penicillium, PANB1—Pan Bacteria1, and PANB3—Pan Bacteria 3. PANB1 include Actinobacteria, Bacteroidetes, Euryarchaeota, Firmicutes, Fusobacteria, Proteobacteria, and Tenericutes. In addition, Pan Bacteria 3 detected species within the phylum Spirochaetes (Table 1). The interpretation of the real-time PCR results was done as per the Kit protocol (16 ). A CT value of 10–20 means high load of bacteria, which indicates that the microorganism could be a pathogen rather than commensal. A CT value of 20–30 means medium load and 30–40 means low load, indicating commensal rather than pathogen. A total of four samples can be tested in a single run.