Hpi t plus 400w metal

Seeds of Ocimum basilicum (Lot 8CJ311048, SC AGROSEL SRL, Romania) were sown in 1 L plastic pots filled with commercial potting soil with nutrients (Biolan Oy, Finland) and grown at 12 h light period under a light intensity of 250 μmol m⁻² s⁻¹ at the level of plants (Philips HPI/T Plus 400W metal halide lamps) as described before (Copolovici and others 2012 (link)). Temperature was maintained at 24°C day/20 °C night and humidity at 60–70%, and plants were watered daily to field capacity. Plants with about 10 inflorescences were used for the experiments. An individual inflorescence with 20 small flowers was carefully detached from the plant in the morning at 10:00, placed in a flask filled with distilled water, and transported to the laboratory for volatile analysis.

One-year-old B. pendula seedlings were collected from
the campus of the Estonian University of Life Sciences
(58˚23’34”N, 26˚41’23”E) and
transplanted into 2 L plastic pots filled with a 1:1:1 mixture of commercial
garden soil (N:P:K=10:8:16, Kekkilä Group, Finland), sand (AS Silicate,
Estonia) and vermiculite (Schetelig Group, Finland). All seedlings were kept in
a plant growth room at 21°C under 12 h light period with the light
intensity of 600 μmol m^-2 s^-1 at the level of
plants (Philips HPI/TPlus 400 W metal halide lamps). Plants were watered twice a
week to soil field capacity.