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Medium matrigel

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Medium-Matrigel is a soluble basement membrane extract that is derived from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It provides a reconstituted basement membrane environment that supports cell attachment, migration, differentiation, and proliferation.

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Lab products found in correlation

Scid mice, by Jackson ImmunoResearch (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Female swiss nu nu mice, by Charles River Laboratories (1 mentions) Nod cb17 prkdcscid j, by Jackson ImmunoResearch (1 mentions) Cell culture insert, by Avantor (1 mentions) Biocoat matrigel invasion inserts, by BD (1 mentions) Cell culture insert, by BD (1 mentions)

Spelling variants of this product

Matrigel (17210 citations) Matrigel culture medium (2 citations) GFR Matrigel™/culture medium (2 citations)

9 protocols using medium matrigel

1

Murine B-Cell Lymphoma Model with Human CD20

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The murine B-cell lymphoma line 38C13 expressing human CD20 (38C13-huCD20) was previously described.(25 (link)) Both 38C13 and 38C13-huCD20 were cultured in RPMI 1640 (Life Technologies) supplemented with 50 μM 2-mercaptoethanol and 10% FBS.
Protocols for all animal studies were approved by the University of California Los Angeles (UCLA) Animal Research Committee. 6- to 8-week old female SCID mice were purchased from Jackson Laboratory. Allografts were established by injecting 0.5×106 cells in 1:1 medium:Matrigel (BD Bioscience) subcutaneously into the shoulder region and were allowed to grow for 5–8 days. Human CD20 transgenic mice (hCD20TM) have been described previously,(16 (link)) and were backcrossed onto BALB/c backgrounds and genotypes confirmed by polymerase chain reaction (PCR).
Zettlitz K.A., Tavaré R., Knowles S.M., Steward K.K., Timmerman J.M, & Wu A.M. (2017). ImmunoPET of malignant and normal B cells with 89Zr- and 124I-labeled obinutuzumab antibody fragments reveals differential CD20 internalization in vivo. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(23), 7242-7252.
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2

Evaluating Novel Combination Therapies for Breast Cancer

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All experimental procedures were conducted in compliance with recommendations issued by the Comite d’Ethique Regional Languedoc-Roussillon - Comite d’Ethique pour l’Experimentation Animale and the Guidelines for the welfare of animals in experimental neoplasia. Female Swiss nu/nu mice (Charles River Laboratories) were housed under clean room conditions in sterile individually ventilated cages. Animals received sterile irradiated chow and water ad libitum.
Ten million SUM159 cells were inoculated subcutaneously in 150 μl of 50:50 medium/matrigel (BD biosciences) in the right hind flank region of 6-week old mice. When tumours reached approximately 100 mm3 animals were randomly assigned into five groups of 10 mice. Mice were then administered with (a) vehicle or (b) oral teriflunomide at a dose of 5 mg/kg once a day for 28 days, (c) 7.5 mg/kg PF477736 twice daily (6-hour interval) by i.p injections on days 2, 9, 16 and 23, (d) teriflunomide and PF477736 according to the same schedules as single regimens and (e) 20 mg/kg paclitaxel by i.p injection on days 1, 8 and 15. Animals were weighed twice a week and tumour volumes were calculated by caliper measurements once every three days using the following formula: V = (length × width × height × π/6). Animals were euthanised when tumour burden reached 1500 mm3.
Arnould S., Rodier G., Matar G., Vincent C., Pirot N., Delorme Y., Berthet C., Buscail Y., Noël J.Y., Lachambre S., Jarlier M., Bernex F., Delpech H., Vidalain P.O., Janin Y.L., Theillet C, & Sardet C. (2017). Checkpoint kinase 1 inhibition sensitises transformed cells to dihydroorotate dehydrogenase inhibition. Oncotarget, 8(56), 95206-95222.
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3

Teratoma Formation Assay for Engineered iPSCs

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All animal procedures were conducted under the Laboratory Animals Welfare Act, the Guide for the Care and Use of Laboratory Animals, and the Guidelines and Policies for Rodent Experimentation provided by the Institutional Animal Care and Use Committee of the College of Medicine of the Catholic University of Korea. This study was approved by the Institutional Review Board of the Catholic University of Korea (CUMC‐2016–0291–02). Immunodeficient nude mice (NOD/CB17-Prkdcscid/J) were purchased from Jackson Laboratories, USA. Wild-type iPSCs and each HLA-B-engineered iPSC clone (1 × 106 cells/clone) were suspended in 30 μl of medium-Matrigel (BD) mixture (DMEM/F12 medium: Matrigel 1:1). The cell mixtures were injected into subcutaneous and testis capsules in 7-week-old NOD mice32 . Eight weeks after injection, when the size of the teratoma of each iPSC clone reached 15 mm in diameter, the teratomas were fixed with 4% paraformaldehyde and processed for hematoxylin and eosin staining for histological evaluation.
Jang Y., Choi J., Park N., Kang J., Kim M., Kim Y, & Ju J.H. (2019). Development of immunocompatible pluripotent stem cells via CRISPR-based human leukocyte antigen engineering. Experimental & Molecular Medicine, 51(1), 3.
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4

Investigating the Role of IRF6 in Subcutaneous Tumor Growth

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A total of four, 6-week-old, athymic nude mice (BALB/cByJNarl) mice were obtained from the National Laboratory Animal Center, Taiwan. UMUC3 cells (1 × 106 for subcutaneous injection) transfected with pcDNA3.1/IRF6 or pcDNA3.1 were re-suspended in 0.1 mL of medium/Matrigel (BD Bioscience, San Jose, CA) mixture (1:1). Cell suspension was injected subcutaneously into the flank of each mouse (day 0). Tumor size was measured daily with calipers in length (L) and width (W). Tumor volume was calculated using the formula (L × W2/2). At the end of experiment, all mice were sacrificed by cervical dislocation. All mice were kept under specific pathogen-free conditions using a laminar airflow rack, with free access to sterilized food and autoclaved water. All experiments were approved by the Animal Experimentation Ethics Committee of the National Chung Cheng University, Taiwan. This study was performed in accordance with the approved guidelines and regulations of National Chung Cheng University.
Jou Y.C., Lin G.L., Lin H.Y., Huang W.H., Chuang Y.M., Lin R.I., Chen P.C., Wu S.F., Shen C.H, & Chan M.W. (2021). Cyproheptadine, an epigenetic modifier, exhibits anti-tumor activity by reversing the epigenetic silencing of IRF6 in urothelial carcinoma. Cancer Cell International, 21, 226.
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5

Chorioallantoic Membrane Scaffold for Cell Culture

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A silicone ring was placed in the centre of the chorioallantoic membrane (CAM) of fertilized chicken eggs (Lohmann LSL-Classic, Lohmann Tierzucht, Germany) on the 8th day of incubation. Immediately afterwards, HepG2 cells (106; ATCC®: HB-8065™) were dispersed in 20 μL of a medium-Matrigel (BD Biosciences, USA) solution (1 : 1 volume ratio) and were placed within the ring.
Diesinger T., Lautwein A., Bergler S., Buckert D., Renz C., Dvorsky R., Buko V., Kirko S., Schneider E., Kuchenbauer F., Kumar M., Günes C., Genze F., Büchele B., Simmet T., Haslbeck M., Masur K., Barth T., Müller-Enoch D., Wirth T, & Haehner T. (2021). A New CYP2E1 Inhibitor, 12-Imidazolyl-1-dodecanol, Represents a Potential Treatment for Hepatocellular Carcinoma. Canadian Journal of Gastroenterology & Hepatology, 2021, 8854432.
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6

Quantifying PC3 Cell Invasion

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After transfection, the invasive behavior of PC3 cells was measured using the BD BioCoat Matrigel Invasion inserts [29 (link)]. Cell culture inserts (VWR International, Bridgeport, NJ) were coated with 50 μl of 1:4 Matrigel/Medium dilutions (BD Sciences, San Jose, CA) and allowed to solidify at 37°C for 1 hr. Cells were resuspended (5.0 × 104 cells/ml) in MEM and 0.1% FBS and 500 μl of cell suspension was added to each insert. Chemoattractant solutions were made as described above with TGF-β1 and EGF into MEM supplemented with 0.1% FBS. Matrigel and non-invading cells were removed by scrubbing. Invading cells in the membrane were fixed in 3.7% paraformaldehyde and stained with DAPI. Pictures were taken from five different fields for average number of invading cells to be determined. The results were expressed as an invasive index defined as: the average number of cells per field for test substance/the average number of cells per field for the medium control. The experiments were conducted at least three times using independent cell preparations.
Barrett C.S., Millena A.C, & Khan S.A. (2016). TGF-β Effects on Prostate Cancer Cell Migration and Invasion Require FosB. The Prostate, 77(1), 72-81.
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7

Evaluating Herbal Treatment for H22 Xenografts

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Male SPF imprinting control region (ICR) mice (22-25 g) were purchased from the Guangdong Animal Center (Guangzhou, Guangdong, China). Mice were inoculated subcutaneously with 5 × 106 H22 cell suspension in Matrigel/medium (BD Biosciences) on the back. The mice were randomly divided into 2 groups (n = 10 for each group), that is, vehicle group and FZQJ group. Mice in the vehicle group were given saline, and those in FZQJ group FZQJ granules 5.4 g/kg/d for 7 days. Another 10 mice without H22 cells served as control. At day 7, all mice were sacrificed and peripheral blood was harvested. Tumors were dissected and weighed.
Chen X., Cao Z., Zhang Y., Li J., Wang S., Du J, & Liao L. (2016). Fuzheng Qingjie Granules Inhibit Growth of Hepatoma Cells via Inducing Mitochondria-Mediated Apoptosis and Enhancing Immune Function. Integrative Cancer Therapies, 16(3), 329-338.
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8

Evaluation of LF and NT5DC3 in Diabetes and Tumor Models

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The animal experiment comprised two parts. Part 1: 15 BALB/c mice were randomly divided into three groups, i.e., a control group (administered PBS), an LF group (administered 250 mg/kg b.w. of LF), and an NT5DC3 protein group (treated with 50 mg/kg b.w. of NT5DC3 injected via the tail vein), n = 5. Part 2: 15 BALB/c mice were fed a high-fat diet for 30 d, while their blood glucose was monitored to ensure the successful construction of the diabetic model. Then, the diabetic mice were assigned to three groups: a control group (administered PBS), an LF group (administered 250 mg/kg b.w. of LF), and an NT5DC3 protein group (treated with 50 mg/kg b.w. of NT5DC3 injected via the tail vein), n = 5.
To construct the tumor model, HT29 cells were cultivated in 150 μL of Matrigel medium (BD) and injected into the backs of the mice. When the tumors reached 100–120 mm3, the mice were treated with PBS, LF or NT5DC3. At the end of the experiment, the mice were sacrificed, and the tumor volumes were measured.
The animal experiments were approved by the Ethics Committee of the Chinese Academy of Agricultural Sciences (Beijing, China; permission number: IAS2020-90).
Li H., Yao Q., Li C., Fan L., Wu H., Zheng N, & Wang J. (2022). Lactoferrin Inhibits the Development of T2D-Induced Colon Tumors by Regulating the NT5DC3/PI3K/AKT/mTOR Signaling Pathway. Foods, 11(24), 3956.
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9

Invasion and Migration Assay for Transfected Cells

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The invasive and migration behavior of transfected cells (2~6 × 104) were washed with 1 × PBS, resuspended in DMEM with 0.2% FBS, and added to the cell culture inserts (24 wells; 8 μm pore, BD Sciences, San Jose, CA). For invasion assay, cell culture inserts were coated with 100 μl of 1:10 Matrigel/Medium dilutions (BD Sciences) and allowed to solidify at 37 °C for 1 h before seeding cells. The medium in the lower compartment was supplemented with TGF-β or 10% FBS. After 16 h, the insert was carefully taken out, fixed by 5% glutaraldehyde for 10 min, and stained with crystal violet. The number of migrating and invasive cells was counted under a microscope. Different views were randomly chosen, and averages were counted.
Chang C.C., Huang Y.S., Lin Y.M., Lin C.J., Jeng J.C., Liu S.M., Ho T.L., Chang R.T., Changou C.A., Ho C.C, & Shih H.M. (2018). The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis. Scientific Reports, 8, 9786.
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