Animals were anesthetized with 3% isoflurane (Butler Schein Animal Health, Dublin, Ohio), delivered at a rate of 1L/min, and bilaterally OVX using an intra-abdominal approach. Following surgery, animals received intraperitoneal (i.p.) injections of butorphanol (0.5mg/kg; Fort Dodge Animal Health, Fort Dodge, IA) to minimize postoperative pain, and subcutaneous (s.c.) injections of gentamicin (10mg/kg; Pro Labs Ltd., St. Joseph, MO) to minimize risk of infection. Behavioral testing commenced after two weeks of postoperative recovery.
Butorphanol
Manufactured by Zoetis
Sourced in Finland, Denmark
Butorphanol is a synthetic opioid analgesic medication used as a veterinary pharmaceutical product. It serves as a pain reliever and sedative for animals. The core function of Butorphanol is to provide analgesia and sedation in veterinary settings.
Automatically generated - may contain errors
Lab products found in correlation
Zoletil, by Virbac (2 mentions)
Torbugesic, by Zoetis (1 mentions)
Antisedan, by Orion Pharma (1 mentions)
Dexdomitor, by Orion Pharma (1 mentions)
Carprofen, by Pfizer (1 mentions)
Ketamine, by Vedco (1 mentions)
Acepromazine maleate, by Vedco (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic mixture, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Dormosedan, by Zoetis (1 mentions)
Sedivet, by Boehringer Ingelheim (1 mentions)
Busulfan, by Merck Group (1 mentions)
Buprenorphine, by Reckitt Benckiser (1 mentions)
Nmri nude mice, by Taconic Biosciences (1 mentions)
Medetomidine, by Zoetis (1 mentions)
Antisedan, by Zoetis (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
3t mri scanner, by GE Healthcare (1 mentions)
13 protocols using butorphanol
1
Ovariectomy and Post-Operative Care
2
Bravo pH Capsule Placement in Cats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Bravo pH monitoring system (Bravo pH capsule with delivery system; Given Imaging, Duluth, Georgia) was placed using radiographic guidance under sedation as previously described.13 All pH capsules and receivers were calibrated as previously described according to the manufacturer's instructions. The location of each pH capsule was kept consistent in each cat among treatment groups by utilizing the measurements on the capsule delivery device to measure the distance from the maxillary canine teeth to the area of capsule placement in the gastric fundus based on radiographs. One day before the first treatment period (Day 0, baseline) and after an overnight fast, cats were sedated with 10 μg/kg dexmedetomidine (dexdomitor 0.5 mg/mL injection; Orion Pharma, Espoo, Finland) IV and 0.4 mg/kg butorphanol (torbugesic 10 mg/mL injection; Fort Dodge Animal Health, Fort Dodge, Iowa) IV. The cats were placed in right lateral recumbency. The pH capsule then was blindly introduced transorally into the proximal stomach as previously described.3 , 13 Sedation was reversed with 100 μg/kg atipamezole (Antisedan 5 mg/mL injection; Orion Pharma, Espoo, Finland) IM after pH capsule placement. The pH capsule placement was repeated in the same manner for each treatment.
3
Feline Neutering: Anesthetic Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cats were fasted for 12 h (water was available ad libitum) prior to neutering by a standard open technique [14] . Cats received 0.3 mg/kg butorphanol (Fort Dodge Animal Health, Fort Dodge, IA), 0.05 mg/kg acepromazine maleate (Vedco, Inc., St. Joseph, MO), and 0.02 mg/kg atropine sulfate (Vedco, Inc., St. Joseph, MO) subcutaneously 30 min before induction. Cats were then induced with 2–3 mg/kg ketamine (Vedco, Inc., St. Joseph, MO) and 0.25 mg/kg diazepam (Hospira, Inc., Lake Forest, IL) intravenously. Additional isoflurane (Vet One, India) delivered by inhalation was used if needed. A subcutaneous dose of 1.5 mg/kg carprofen (Pfizer, Inc., Madison, NJ) was given post operatively and repeated 24 h later.
4
Isolation of Muscle-Derived Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Skeletal muscle samples (about 0.05 g) for cell isolation were obtained from musculus gracilis during general anesthesia induced by administration of xylazine (10 mg/kg; Leciva, Prague, Czech Republic), ketamine (40 mg/kg; Spofa, Prague, Czech Republic), and butorphanol (1 mg/kg; Fort Dodge Animal Health, Fort Dodge, IA, USA). Tissue sampling did not impair rats mobility after surgery. Isolation of muscle-derived cells was performed as described by Burdzińska et al. [19 (link)]. The cells were suspended in standard growth medium (GM), DMEM supplemented with 10% (v/v) fetal bovine serum and antibiotic, antimycotic mixture (all components purchased from Invitrogen, Carlsbad, CA, USA). In order to reduce number of fibroblasts in culture, the medium containing nonadherent cells was removed to another dish 24 h after cell seeding (preplating). The first change of culture medium was performed 72 h after isolation. When the culture reached 70% of confluence, cells were harvested by trypsinization (0.25% trypsin and 0.02% EDTA; Invitrogen-Gibco Carlsbad, USA) and reseeded in new dishes in a density of 5 × 103/cm2. Majority of cells were cultured for transplantation whereas part of population were seeded separately to perform in vitro characterization, desmin expression and differentiation potential analysis.
5
Ovariectomy and Behavioral Testing
6
Foal Physical Examination and Sedation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each foal underwent the same, routine physical examination. Grading systems were used to quantify subjective data. Nasal discharge was given a score of 0–3 (Table 1 ).
Thoracic auscultation was scored from 0 to 3 based on type and severity of audible pathology (Table2 ). After physical examination, each foal was sedated with 0.005–0.02 mg/kg Detomidine HCL and 0.01–0.05 mg/kg Butorphanol via intravenous injection (Dormosedan, 10 mg/mL, Zoetis Inc. Butorphanol, Butador, 10 mg/mL, Nutri Biomed) depending on temperament.
Thoracic auscultation was scored from 0 to 3 based on type and severity of audible pathology (Table
7
Experimental Infection of Foals with R. equi
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Foals from Group 1 and 2 were experimentally infected at age 21 days with 1 x 106 CFU of live R. equi (strain EIDL 5–331, the same strain used for the vaccine). Prior to experimental infection with R. equi, each foal’s lungs were evaluated by auscultation and thoracic ultrasonography to document absence of pre-existing lung disease. Foals were sedated using intravenous injection of romifidine (0.8 mg/kg; Sedivet, Boehringer-Ingelheim Vetmedica, Inc., St. Joseph, MO, USA) and butorphanol (0.02 mg/kg; Zoetis, Florham Park, New Jersey, USA) to facilitate endoscopy. An aseptically-prepared, videoendoscope with outer diameter of 9-mm was inserted via the nares into the trachea and passed to the bifurcation of the main-stem bronchi. A 40-mL suspension of virulent EIDL 5–331 R. equi containing approximately 1 x 106 viable bacteria was administered transendoscopically, with 20 ml infused into the right mainstem bronchus and 20 ml into the left mainstem bronchus. The channel was flushed twice with 20 ml of air after each 10 ml bacterial infusion.
8
Busulfan-induced Infertile Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nude mice (Naval Medical Research Institute (NMRI)-NU, Charles River, Denmark) were housed in groups, fed pellets and water ad libitum, and kept under controlled 12-hour light/12-hour dark cycles at 20-22°C. At eight weeks of age, each testis of mice was injected with 80 μg busulfan (B2635, Sigma-Aldrich) to eliminate endogenous spermatogenesis. The busulfan was dissolved in dimethyl sulfoxide (DMSO) and delivered in a volume of 20μl through two different sites (24 (link), 31 (link)). Xenotransplantation was performed 4-5 weeks after busulfan treatment. Both injection and xenotransplantation were performed under anesthesia using Zoletil (Virbac, France), xylazin (Scanvet, Denmark), and butorphanol (Zoetis, New Jersey). Post-operative analgesia was provided by use of buprenorphine (Reckitt Benckiser; England, UK) and carprofen (Norbrook, England, UK). Following xenotransplantation, mice were single-housed until euthanasia. Euthanasia was done by cervical dislocation.
9
Ovarian Xenotransplantation in NMRI Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ten female immunodeficient Naval Medical Research Institute (NMRI)-NUDE mice aged 6–7 weeks were purchased from Taconic, Denmark. Mice were housed in groups, fed pellets and water ad libitum, and kept under controlled 12-h light/12-h dark cycles at 20–22 °C. One week after arrival the mice were anesthetized using Zoletil (Virbac, France), xylazin (Scanvet, Denmark), and butorphanol (Zoetis, New Jersey) before they were ovariectomized to increase endogenous FSH levels. Post-operative analgesia in the form of buprenorphine (Temgesic, Indivior UK Limited, UK) and carprofen (Norodyl, ScanVet, Denmark) was used. Two weeks after ovariectomy either six (4-week study) or eight pieces (3, 6, 10 days study) of human ovarian cortex were transplanted subcutaneously to the back of each mouse. After surgery the mice were single housed for at least 1 week. Euthanasia was performed by cervical dislocation, upon graft retrieval.
10
Delta Variant SARS-CoV-2 Infection in Cats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Details regarding the B.6.617.2 delta variant infection protocols have been previously published [13 (link)]. Briefly, cats were anesthetized with ketamine (4 mg/kg) (Covetrus, Dublin, OH, USA), dexmedetomidine (20 µg/kg) (Orion, Espoo, Finland), and butorphanol (0.4 mg/kg) (Zoetis, Gilrona, Spain) intramuscularly. Cats were inoculated at the level of the distal trachea using 1 mL of Dulbecco’s Modified Eagle Medium (DMEM) (GibcAqo, Carlsbad, CA, USA), containing 1.26 × 106 TCID50 of SARS-CoV-2 isolate B.6.617.2 (delta), as previously described [13 (link)]. Sham-inoculated cats were administered 1 mL of inoculation media. At day 4 and day 12 post-infection, cats infected with delta SARS-CoV-2 (n = 12 per time-point) and sham-infected control cats (n = 3 per timepoint) were anesthetized, and then humanely euthanized (pentobarbital > 80 mg/kg) for tissue collection. Following euthanasia, necropsy tissues including cranial lung, left ventricle, gastrocnemius, and pancreas were collected. Tissues were divided and either frozen at −80 °C or collected into tissue cassettes and fixed in 10% neutral-buffered formalin for 5 days prior to transferring to 70% ethanol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!