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11 protocols using acetonitrile

1

Analytical Protocol for Pesticide and Chemical Quantification

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DM, cypermethrin, fenvalerate, fenpropathrin, lambda-cyhalothrin, and bifenthrin (>98% purify) samples were purchased from Jiangsu Rongde Reagent Factory (Zhenjiang, China). Green tea, black tea, and tieguanyin (dried tea) were purchased from Fujian Anxi Country Xiyun Tea Industry Co., Ltd. (Quanzhou, China). Black tea beveragewas purchased from Nongfu Spring Jiande New Anjiang Beverage Co., Ltd. (Hangzhou, China). Furthermore, AM, ethylene glycol dimethacrylate (EGDMA), and azobisisobutyronitrile (AIBN) were bought from Aladdin Industrial Corporation (Shanghai, China). Absolute ethanol and acetic acid were obtained from Guangdong Guanghua Technology Co., Ltd. (Shantou, China). Methanol was purchased from Guangzhou Dongjiang Chemical Plant (Guangzhou, China). Acetone-D6 was purchased from Merck Chemical Technology Co., Ltd. (Shanghai, China). Sodium lauryl sulfate were purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. (Shanghai China). Dibutyl phthalate was purchased from Tianjin Bodi Chemical Co., Ltd. (Tianjin China). Hydroxyethyl cellulose (HEC) was purchased from Guangzhou Qingfeng Chemical Co., Ltd. (Guangzhou China). Chloroform, acetonitrile, and octanol were purchased from Tianjin Damao Chemical Reagent Factory (Tianjin China). N-hexane and acetone were purchased from Guangdong Guanghua Technology Co., Ltd. (Shantou, China).
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2

Characterization of DOM Sources

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Anthracene (99.0%) and pyrene (97.0%) were from Shanghai McLean. Sodium hydroxide (NaOH) and acetonitrile were from Tianjin Damao Chemical Reagent Factory for analytical purity. Deionized water was prepared with Millipore Milli-Q system. Suwannee River fulvic acid Standard II (SRFA), Upper Mississippi River NOM (UMRN) are derived from water source DOM, lliott Soil Humic Acid Standard IV (ESHA), Leonardite Humic Acid Standard (LHA) are from land source DOM which were purchased from the International Humic Acid Association.
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3

Derivatization Reagents and Solvents

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d-Xylose (Batch No. 20160302) was purchased from Tianjin Kaixin Chemical Industry Co., LTD. Phloroglucinol, glacial acetic acid, chloral hydrate, acetonitrile (chromatographically pure), methanoic acid (chromatographically pure), ammonium hydroxide, n-butyl alcohol, methyl alcohol (chromatographically pure) and acetone were all provided by Damao Chemical Reagent Factory (Tianjin, China; Batch Nos. 20190416, 20151123, 20180716, 20200616, 20200713, 20200702, 20200316, 20201225 and 20150501). Dinitrofluorobenzene (DNFB) was provided by Tokyo Chemical Industry Co. LTD (Batch No. VP2UE-RE). DNFB solution was prepared in 1% solution with 1:1 acetone-sesame oil solution before application.
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4

Detailed Reagent Procurement Protocol

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Vanillin was purchased Shanghai Sangon Biological Engineering Technology (Shanghai, China). o-Toluidine and N, N-dimethylformamide were purchased from Shanghai Aladdin Bio-Chem Technology Co., LTD (Shanghai, China). Sodium citrate tribasic dihydrate and Tris were purchased from Sigma-Aldrich (Steinheim, Germany). Glycine was purchased from GenStar Biosolutions Co., Ltd. (Beijing, China). Citric acid, disodium hydrogen, HCl, NaOH, Na2HPO4 and acetic acid were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). Acetonitrile was purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). n-Hexane was purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China). Other chemicals were purchased from standard commercial sources and were of analytical grade purity.
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5

Synthesis of Cadmium Nitrate Complex

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Cd(NO3)2·4H2O (99%) was purchased from Shanghai Macklin Biochemical Co., Ltd. Thiourea (99%) and (±)1-phenylethanol (98%) were purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. Ethylenediamine (99%) and acetonitrile (99%) were purchased from Damao Chemical Reagent Factory. All the reagents were used as received without further purification.
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6

Febuxostat Synthesis and Characterization

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Deionized water (homemade), febuxostat (purity ≥98%, Shanghai Dibo Biotechnology Co., Ltd.) anhydrous ethanol (Tianjin Xinbote Chemical Co. Ltd.), urea (Tianjin Yongsheng Fine Chemical Co. Ltd.), acetonitrile (Tianjin Damao Chemical Reagent Factory), and potassium stannate trihydrate were purchased from Aladdin Industrial Company (Shanghai, China). 3-Aminopropyltriethoxysilane (APTES) was purchased from Aladdin Industrial Corporation (Shanghai, China). Hematoxylin-eosin/HE staining kits were purchased from Beyotime Biotechnology Co. Chitosan, dimethicone, tetrahydrofuran, formic acid, and heparin were purchased from Sinopharm Chemical Reagent Co. Ltd.
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7

Sulfadiazine-based Analytical Assay

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Sulfadiazine (SDZ), ferric citrate, p-benzoquinone and l-histidine were analytically pure and purchased from Shanghai Maclean Biochemical Technology Co., China. Methanol (MeOH), tert-butanol, hydrochloric acid, sodium hydroxide, potassium peroxymonosulfate (PMS) and anhydrous ethanol were analytically pure, and acetonitrile was chromatographically pure, purchased from Tianjin Damao Chemical Reagent Co., China. Water used for the mobile phase in liquid chromatography was ultrapure water, and the experimental water was deionized.
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8

Synthesis of DMNQ Compound

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An amount 5.5 g cerium (IV) diammonium nitrate (Tianjin Damao Chemical Reagent Co., Ltd.) was dissolved in 250 ml H2O and added drop-wise to a solution of 15 g 1,4,5,8-tetramethoxynaphthalene in 160 ml trichloromethane (Tianjin Damao Chemical Reagent Co., Ltd.) and 480 ml acetonitrile (Tianjin Damao Chemical Reagent Co., Ltd.). This mixture was stirred at 25˚C for 1.5 h. The key compound DMNQ (Fig. 1A-c) was obtained by this oxidation reaction.
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9

Britton–Robinson Buffer Solutions Preparation

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Britton–Robinson buffer solutions (B–R) with different pH values were prepared by titrating 0.1 M sulfuric acid, phosphoric acid, boric acid and 0.1 M sodium hydroxide. Quinine sulfate, 3-aminopropyltriethoxysilane (APTES), cysteine, balofloxacin, clinafloxacin, sparfloxacin, norfloxacin, pefloxacin mesylate, and enrofloxacin were purchased from Aladdin Reagent Company. Methotrexate, methionine, phenylalanine, and l-cysteine were purchased from Macklin Reagent Company. Methanol, ethanol, isopropanol, acetonitrile, dimethyl sulfoxide, l-glutamate, d-fructose, glucose, l-glutamic acid, KI, sulfuric acid, phosphoric acid, and boric acid were purchased from Tianjin Damao Chemical Reagent Factory. K2CO3, KCl, BaCl2, CuCl2, FeCl3, MnCl2, CrCl3, CaCl2, and BeCl2 were all utilized as analytically pure reagents, and purchased from Kelong Reagent Factory. KF, ZnSO4, Al2(SO4)3, Mg2SO4, PbCl2, and Cd2SO4 were all used as analytically pure reagents and purchased from Guanghua Reagent Company. Ultrapure water was used in all the experiments.
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10

Synthesis of Aromatic Compounds

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3,3′-Diaminobenzidine, glutaric acid, and bromopentane (n-C5H11Br) were purchased from Energy chemical technology (Shanghai) Co. Ltd. Polyphosphoric acid (PPA) was purchased from Macklin biochemical technology Co. Ltd. All soluble metal ion salts and sodium hydroxide (NaOH) are purchased from Guangzhou Chemical reagent factory. All the nitroaromatic compounds purchased from Guangzhou Chemical reagent factory. Tetrahydrofuran (THF), acetonitrile (MeCN), anhydrous ethanol (EtOH), dimethyl formamide (DMF), dichloro-methane (DCM), and dimethyl sulfoxide (DMSO) were purchased from Tianjin Damao chemical reagent factory. DMSO-d6 and CDCl3 were purchased from Energy chemical technology (Shanghai) Co. Ltd. All these reagents were used without further purification.
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